ArticleJournal of clinical microbiology1996
Genotyping of outbreak-related and sporadic isolates of Clostridium difficile belonging to serogroup C.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 42 citations in OpenAlex.
- DNA microarray-based PCR ribotyping of Clostridium difficile.Journal of clinical microbiology · 2015Article
- Increased sporulation rate of epidemic Clostridium difficile Type 027/NAP1.Journal of clinical microbiology · 2008Article
- Molecular epidemiology of endemic Clostridium difficile infection and the significance of subtypes of the United Kingdom epidemic strain (PCR ribotype 1).Journal of clinical microbiology · 2005Article
- Characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping.Journal of clinical microbiology · 2004Article
- Epidemiology and molecular characterization of Clostridium difficile strains from patients with diarrhea: low disease incidence and evidence of limited cross-infection in a Swedish teaching hospital.Journal of clinical microbiology · 2003Article
- Transcription and analysis of polymorphism in a cluster of genes encoding surface-associated proteins of Clostridium difficile.Journal of bacteriology · 2003Article
- Analysis of Clostridium difficile isolates from nosocomial outbreaks at three hospitals in diverse areas of Japan.Journal of clinical microbiology · 2001Article
- Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.Journal of clinical microbiology · 2000Article
- Comparison of restriction enzyme analysis, arbitrarily primed PCR, and protein profile analysis typing for epidemiologic investigation of an ongoing Clostridium difficile outbreak.Journal of clinical microbiology · 1998Article
- A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates.Journal of clinical microbiology · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serogroup C of Clostridium difficile is the serogroup most frequently related to outbreaks. Fifty-six toxigenic serogroup C isolates of C. difficile were genotyped by ribotyping PCR (ribo-PCR), random amplified polymorphic DNA (RAPD) assay, and pulsed-field gel electrophoresis (PFGE). Thirty-five of the 56 isolates were recovered from four unrelated outbreaks (Belgium, 1987, 1992, and 1995; France, 1992 to 1993) 7 derived from a spatiotemporal cluster in Cotonou, Benin (1992), and 14 were sporadic isolates. The serogroup C reference strain, also isolated during an outbreak (Belgium, 1983), was genotyped too. Ribo-PCR, the RAPD assay, and PFGE generated 2, 5, and 11 major genotypes, respectively. Combination of the three methods finally yielded 13 general types, although ribo-PCR did not play any role in enhancing resolution. Three general types were recovered from all the isolates from the five outbreaks and the cluster, with two types being predominant. The 14 sporadic serogroup C isolates were divided into 11 overall genotypes. These results indicate that genotyping methods, and more particularly the combination of the RAPD assay and PFGE, can resolve genetic diversity within toxigenic, serogroup C C. difficile strains. Also, this study suggests that outbreak-related serogroup C strains are limited to a few genetically stable and apparently very widely (internationally and intercontinentally) distributed genotypes.
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