Trial reportJournal of clinical microbiology1996
Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma.
Trial report in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Obtaining unacceptable results in assays for quantitation of human immunodeficiency virus type 1 RNA in plasma samples.Journal of clinical microbiology · 2000Trial
- HIV Viral Load Testing in the South African Public Health Setting in the Context of Evolving ART Guidelines and Advances in Technology, 2013-2022.Diagnostics (Basel, Switzerland) · 2023Article
- Article
- SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA).Lab on a chip · 2018Article
- Thirty-day postoperative mortality among individuals with HIV infection receiving antiretroviral therapy and procedure-matched, uninfected comparators.JAMA surgery · 2015Article
- Current practices in laboratory monitoring of HIV infection.The Indian journal of medical research · 2011Review
- International multicenter study to assess a panel of reference materials for quantification of simian immunodeficiency virus RNA in plasma.Journal of clinical microbiology · 2010Article
- Principles and applications of polymerase chain reaction in medical diagnostic fields: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2009Article
- HIV-1 viral load assays for resource-limited settings.PLoS medicine · 2006Review
- Molecular and diagnostic clinical virology in real time.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2004Review
- Clinical virology in real time.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2002Review
- External quality assessment program for qualitative and quantitative detection of hepatitis C virus RNA in diagnostic virology.Journal of clinical microbiology · 2002Article
- Viral dynamics during structured treatment interruptions of chronic human immunodeficiency virus type 1 infection.Journal of virology · 2002Article
- Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses.Journal of clinical microbiology · 2001Article
- Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0.Clinical and diagnostic laboratory immunology · 2001Article
- Single rapid real-time monitored isothermal RNA amplification assay for quantification of human immunodeficiency virus type 1 isolates from groups M, N, and O.Journal of clinical microbiology · 2001Article
- Quantitation of human immunodeficiency virus type 1 RNA in different biological compartments.Journal of clinical microbiology · 2000Article
- Antigen-driven CD4+ T cell and HIV-1 dynamics: residual viral replication under highly active antiretroviral therapy.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Design and evaluation of a human immunodeficiency virus type 1 RNA assay using nucleic acid sequence-based amplification technology able to quantify both group M and O viruses by using the long terminal repeat as target.Journal of clinical microbiology · 1999Article
- Comparison of levels of human immunodeficiency virus type 1 RNA in plasma as measured by the NucliSens nucleic acid sequence-based amplification and Quantiplex branched-DNA assays.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three procedures for the quantification of human immunodeficiency virus type 1 (HIV-1) RNA from plasma were compared at three laboratories. The comparison involved the Quantiplex branched DNA assay (version 1.0) by Chiron Diagnostics, the NASBA-QT assay by Organon Teknika, and the Amplicor Monitor assay by Roche Molecular Systems. The laboratories performed each of the three assays with the same sets of reconstructed HIV-1-infected human plasma samples, cross-sectionally collected clinical plasma samples and longitudinally collected plasma samples from patients starting zidovudine therapy. Analysis of the reconstruction panel results for interlaboratory variation demonstrated that no laboratory differences in results were detected for any of the assays. A comparison of the reproducibilities of duplicate samples analyzed by batch and in separate assay runs demonstrated that the reproducibilities of the test results were similar within one assay and appeared to be independent of the HIV-1 concentration. The best reproducibility was obtained with the Quantiplex assay, but all three assays demonstrated equal reliability, which was independent of batched or unbatched analysis of replicate samples. Differences in the absolute concentrations calculated were observed for the assays, in particular in the analysis of reconstructed samples. In all assays, similar changes in plasma HIV-1 RNA concentrations were determined for longitudinally collected clinical samples.
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