ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1996
Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.).
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 50 citations in OpenAlex.
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Multiple mating and a low incidence of cuckoldry for nest-holding males in the two-spotted goby, Gobiusculus flavescens.BMC evolutionary biology · 2009Article
- Flow cytogenetics and plant genome mapping.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
- Introduction: chromosomes go with the flow.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
- Development of flow cytogenetics and physical genome mapping in chickpea (Cicer arietinum L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Article
- Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant DNA libraries were constructed for seven chromosome types isolated from two translocation lines of field bean (Vicia faba L.) with reconstructed karyotypes. The chromosomes were selected so that the set of libraries covers the whole V. faba genome more than once. Individual chromosome types were highly purified by flow sorting, and their DNA was amplified by degenerate oligonucleotide-primed (DOP) polymerase chain reaction (PCR) and cloned into a plasmid vector. The choice of restriction site present in PCR primer and refinement of cloning protocol resulted in high cloning efficiency and allowed generation of libraries consisting of about 10(5) clones from 250 or 1000 sorted chromosomes. The insert size ranged between 50 and 2200 bp and the mean length estimated in individual libraries varied between 310 and 487 bp. Hybridization of cloned fragments with labelled genomic DNA showed that about 60% of inserts represented unique or low-copy sequences. The suitability of the libraries for genome mapping was demonstrated by isolation of clones containing microsatellite motifs.
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