ArticleNucleic acids research1996
Inverse splicing of a discontinuous pre-mRNA intron generates a circular exon in a HeLa cell nuclear extract.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- CircRNA: a rising therapeutic strategy for lung injury induced by pulmonary toxicants.Archives of toxicology · 2024Review
- Circular RNA-mediated miRNA sponge & RNA binding protein in biological modulation of breast cancer.Non-coding RNA research · 2024Review
- The role and therapeutic potential of macrophages in the pathogenesis of diabetic cardiomyopathy.Frontiers in immunology · 2024Review
- Cardiovascular complications of diabetes: role of non-coding RNAs in the crosstalk between immune and cardiovascular systems.Cardiovascular diabetology · 2023Review
- Review
- circRNA: A New Biomarker and Therapeutic Target for Esophageal Cancer.Biomedicines · 2022Review
- The Functional Roles and Regulation of Circular RNAs during Cellular Stresses.Non-coding RNA · 2022Review
- Review
- The design and synthesis of circular RNAs.Methods (San Diego, Calif.) · 2021Review
- Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts.Nucleic acids research · 2020Article
- Clinical and functional significance of circular RNAs in cytogenetically normal AML.Blood advances · 2020Article
- Functions of Circular RNAs in Regulating Adipogenesis of Mesenchymal Stem Cells.Stem cells international · 2020Review
- Circular RNAs in Cardiac Regeneration: Cardiac Cell Proliferation, Differentiation, Survival, and Reprogramming.Frontiers in physiology · 2020Review
- The Nefarious Nexus of Noncoding RNAs in Cancer.International journal of molecular sciences · 2018Review
- A 360° view of circular RNAs: From biogenesis to functions.Wiley interdisciplinary reviews. RNA · 2018Review
- Molecular roles and function of circular RNAs in eukaryotic cells.Cellular and molecular life sciences : CMLS · 2018Review
- Article
- Circular RNA expression profiles and features in human tissues: a study using RNA-seq data.BMC genomics · 2017Article
- Circular RNA - New member of noncoding RNA with novel functions.Experimental biology and medicine (Maywood, N.J.) · 2017Review
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently reported the first example of inverse splicing of a eukaryotic pre-mRNA intron using a whole cell extract from the yeast Saccharomyces cerevisiae. The concomitant circularization of the exon in the course of this splicing reaction gave rise to the hypothesis that the circular RNA species, which had been recently discovered in vivo in mammalian cells, were generated by inverse splicing. Here we report the formation of a circular exon in HeLa cell nuclear extracts by an inverse splicing reaction of the second intron of the human beta-globin gene from a pre-mRNA transcript in which the two intron halves flanked an artificially fused, single exon. Our data demonstrate that the mammalian pre-mRNA splicing system has indeed an intrinsic capability of aligning splice sites in reverse order and that this alignment can be followed by a complete splicing reaction, whereby the discontinuous intron sequences are removed. Thus we propose that circular exons in vivo arise as a result of an inverse splicing reaction following the pairing of a 5' splice site with an upstream 3' splice site and that the frequency of this event is influenced by the presence and strength of other, competing splice sites.
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