ArticleJournal of bacteriology1996
Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Regulation of membrane protein activity by cyclopropane fatty acids in Escherichia coli lipid environment.Communications biology · 2025Article
- NhaR, LeuO, and H-NS Are Part of an Expanded Regulatory Network for Ectoine Biosynthesis Expression.Applied and environmental microbiology · 2023Article
- Morphological Phenotypes, Cell Division, and Gene Expression ofMicroorganisms · 2022Article
- Transcriptional Sequencing Uncovers Survival Mechanisms of Salmonella enterica Serovar Enteritidis in Antibacterial Egg White.mSphere · 2019Article
- Contrasting silencing mechanisms of the same target mRNA by two regulatory RNAs in Escherichia coli.Nucleic acids research · 2018Article
- Article
- Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction.Molecular microbiology · 2017Article
- Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae.PloS one · 2017Article
- Article
- Effect of overexpressing nhaA and nhaR on sodium tolerance and lactate production in Escherichia coli.Journal of biological engineering · 2013Article
- Translational repression of NhaR, a novel pathway for multi-tier regulation of biofilm circuitry by CsrA.Journal of bacteriology · 2012Article
- The cation-responsive protein NhaR of Escherichia coli activates pgaABCD transcription, required for production of the biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine.Journal of bacteriology · 2006Article
- Alkaline pH homeostasis in bacteria: new insights.Biochimica et biophysica acta · 2005Review
- Gene expression profiling reflects physiological processes in salt acclimation of Synechocystis sp. strain PCC 6803.Plant physiology · 2004Article
- NhaR and RcsB independently regulate the osmCp1 promoter of Escherichia coli at overlapping regulatory sites.Journal of bacteriology · 2003Article
- Isolation and characterization of NaCl-sensitive mutants of Caulobacter crescentus.Applied and environmental microbiology · 2003Article
- Chloride, a new environmental signal molecule involved in gene regulation in a moderately halophilic bacterium, Halobacillus halophilus.Journal of bacteriology · 2002Article
- GerN, an endospore germination protein of Bacillus cereus, is an Na(+)/H(+)-K(+) antiporter.Journal of bacteriology · 2001Article
- Transcription of nhaA, the main Na(+)/H(+) antiporter of Escherichia coli, is regulated by Na(+) and growth phase.Journal of bacteriology · 2001Article
- NhaA, an Na(+)/H(+) antiporter involved in environmental survival of Vibrio cholerae.Journal of bacteriology · 2000Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
nhaA encodes an Na+/H+ antiporter in Escherichia coli which is essential for adaptation to high salinity and alkaline pH in the presence of Na+. We used Northern (RNA) analysis to measure directly the cellular levels of nhaA mRNA. NhaR belongs to the LysR family of regulatory proteins. Consistent with our previous data with an nhaA'-'lacZ fusion, NhaR was found to be a positive regulator and Na+ was found to be a specific inducer of nhaA transcription. In the nhaA'-'lacZ fusion, maximal induction was observed at alkaline pH. In contrast, in the nhaA+ strain both the level of nhaA expression and the induction ratio were lower at alkaline pH. This difference may be due to the activity of NhaA in the wild-type strain as NhaA efficiently excreted Na+ at alkaline pH and reduced the intracellular concentration of Na+, the signal for induction. We also showed that although the global regulator rpoS was not involved in nhaA regulation, the global regulator hns played a role. Thus, the expression of nhaA'-'lacZ was derepressed in strains bearing hns mutations and transformation with a low-copy-number plasmid carrying hns repressed expression and restored Na+ induction. The derepression in hns strains was nhaR independent. Most interestingly, multicopy nhaR, which in an hns+ background acted only as an Na+-dependent positive regulator, acted as a repressor in an hns strain in the absence of Na+ but was activated in the presence of the ion. Hence, an interplay between nhaR and hns in the regulation of nhaA was suggested.
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