ArticleMolecular biology of the cell1996
Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 72 citations in OpenAlex.
- Proteome-Wide Discovery of Degradable Proteins Using Bifunctional Molecules.ACS central science · 2025Article
- A proteome-wide yeast degron collection for the dynamic study of protein function.The Journal of cell biology · 2025Article
- The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.The Journal of cell biology · 2024Article
- NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma.Scientific reports · 2023Article
- Fantastic nuclear envelope herniations and where to find them.Biochemical Society transactions · 2018Review
- Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1-Npl4.Nature structural & molecular biology · 2018Article
- Article
- The ESCRT machinery: new roles at new holes.Current opinion in cell biology · 2016Review
- ESCRTs breach the nuclear border.Nucleus (Austin, Tex.) · 2015Article
- The VCP/p97 system at a glance: connecting cellular function to disease pathogenesis.Journal of cell science · 2014Review
- Evolution of mir-92a underlies natural morphological variation in Drosophila melanogaster.Current biology : CB · 2013Article
- 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay.The EMBO journal · 2012Article
- The complexities of p97 function in health and disease.Molecular bioSystems · 2011Review
- A Cdc48p-associated factor modulates endoplasmic reticulum-associated degradation, cell stress, and ubiquitinated protein homeostasis.The Journal of biological chemistry · 2011Article
- Changes in the nuclear envelope environment affect spindle pole body duplication in Saccharomyces cerevisiae.Genetics · 2010Article
- TDP-43 mediates degeneration in a novel Drosophila model of disease caused by mutations in VCP/p97.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution.The Journal of cell biology · 2010Article
- The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.The Journal of cell biology · 2007Article
- Structural insights into the p97-Ufd1-Npl4 complex.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.Genetics · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
To identify components involved in nuclear protein import, we used a genetic selection to isolate mutants that mislocalized a nuclear-targeted protein. We identified temperature-sensitive mutants that accumulated several different nuclear proteins in the cytoplasm when shifted to the semipermissive temperature of 30 degrees C; these were termed npl (nuclear protein localization) mutants. We now present the properties of yeast strains bearing mutations in the NPL4 gene and report the cloning of the NPL4 gene and the characterization of the Np14 protein. The npl4-1 mutant was isolated by the previously described selection scheme. The second allele, npl4-2, was identified from an independently derived collection of temperature-sensitive mutants. The npl4-1 and npl4-2 strains accumulate nuclear-targeted proteins in the cytoplasm at the nonpermissive temperature consistent with a defect in nuclear protein import. Using an in vitro nuclear import assay, we show that nuclei prepared from temperature-shifted npl4 mutant cells are unable to import nuclear-targeted proteins, even in the presence of cytosol prepared from wild-type cells. In addition, npl4-2 cells accumulate poly(A)+ RNA in the nucleus at the nonpermissive temperature, consistent with a failure to export mRNA from the nucleus. The npl4-1 and npl4-2 cells also exhibit distinct, temperature-sensitive structural defects: npl4-1 cells project extra nuclear envelope into the cytoplasm, whereas npl4-2 cells from nuclear envelope herniations that appear to be filled with poly(A)+ RNA. The NPL4 gene encodes an essential M(r) 64,000 protein that is located at the nuclear periphery and localizes in a pattern similar to nuclear pore complex proteins. Taken together, these results indicate that this gene encodes a novel nuclear pore complex or nuclear pore complex-associated component required for nuclear membrane integrity and nuclear transport.
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