ArticleMolecular biology of the cell1996
Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 119 citations in OpenAlex.
- CDC6 as a Key Inhibitory Regulator of CDK1 Activation Dynamics and the Timing of Mitotic Entry and Progression.Biology · 2023Review
- The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint.Open biology · 2022Article
- Control of pre-replicative complex during the division cycle in Chlamydomonas reinhardtii.PLoS genetics · 2021Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.The Journal of biological chemistry · 2012Article
- Chemical visualization of phosphoproteomes on membrane.Molecular & cellular proteomics : MCP · 2012Article
- Regulation of the final stage of mitosis by components of the pre-replicative complex and a polo kinase.Cell cycle (Georgetown, Tex.) · 2011Article
- Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A comprehensive molecular interaction map of the budding yeast cell cycle.Molecular systems biology · 2010Review
- Article
- Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication.The Journal of biological chemistry · 2007Article
- Roles of the CDK phosphorylation sites of yeast Cdc6 in chromatin binding and rereplication.Molecular biology of the cell · 2007Article
- Mitotic Cdc6 stabilizes anaphase-promoting complex substrates by a partially Cdc28-independent mechanism, and this stabilization is suppressed by deletion of Cdc55.Molecular and cellular biology · 2007Article
- ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA.Nature structural & molecular biology · 2005Article
- The role and regulation of the preRC component Cdc6 in the initiation of premeiotic DNA replication.Molecular biology of the cell · 2004Article
- The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation.The Journal of cell biology · 2004Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit.Molecular biology of the cell · 2003Article
- Targeted destruction of DNA replication protein Cdc6 by cell death pathways in mammals and yeast.Molecular biology of the cell · 2002Article
- The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
During purification of recombinant Cdc6 expressed in yeast, we found that Cdc6 interacts with the critical cell cycle, cyclin-dependent protein kinase Cdc28. Cdc6 and Cdc28 can be coimmunoprecipitated from extracts, Cdc6 is retained on the Cdc28-binding matrix p13-agarose, and Cdc28 is retained on an affinity column charged with bacterially produced Cdc6. Cdc6, which is a phosphoprotein in vivo, contains five Cdc28 consensus sites and is a substrate of the Cdc28 kinase in vitro. Cdc6 also inhibits Cdc28 histone H1 kinase activity. Strikingly, Cdc6 interacts preferentially with B-type cyclin/Cdc28 complexes and not Cln/Cdc28 in log-phase cells. However, Cdc6 does not associate with Cdc28 when cells are blocked at the restrictive temperature in a cdc34 mutant, a point in the cell cycle when the B-type cyclin/Cdc28 inhibitor p40Sic1 accumulates and purified p40Sic1 inhibits the Cdc6/Cdc28 interaction. Deletion of the Cdc28 interaction domain from Cdc6 yields a protein that cannot support growth. However, when overproduced, the mutant protein can support growth. Furthermore, whereas overproduction of wild-type Cdc6 leads to growth inhibition and bud hyperpolarization, overproduction of the mutant protein supports growth at normal rates with normal morphology. Thus, the interaction may have a role in the essential function of Cdc6 in initiation and in restraining mitosis until replication is complete.
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