ArticleInfection and immunity1996
Expression and characterization of glutathione peroxidase activity in the human blood fluke Schistosoma mansoni.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Oxidative Stress and Cancer Risk in Schistosomiasis.Oxidative medicine and cellular longevity · 2024Review
- Assessment of antioxidant enzymes during the development of the digenetic trematode parasiteJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica.Parasitology research · 2018Article
- Biochemical properties and vaccine effect of recombinant TPx-3 from Schistosoma japonicum.Parasitology research · 2017Article
- Article
- Curcumin Generates Oxidative Stress and Induces Apoptosis in Adult Schistosoma mansoni Worms.PloS one · 2016Article
- Protective Potential of Antioxidant Enzymes as Vaccines for Schistosomiasis in a Non-Human Primate Model.Frontiers in immunology · 2015Article
- Schistosoma tegument proteins in vaccine and diagnosis development: an update.Journal of parasitology research · 2012Article
- The redox biology of schistosome parasites and applications for drug development.Current pharmaceutical design · 2012Review
- Role of the endogenous antioxidant system in the protection of Schistosoma mansoni primary sporocysts against exogenous oxidative stress.PLoS neglected tropical diseases · 2009Article
- Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).Parasitology · 2009Article
- Nucleic acid vaccination with Schistosoma mansoni antioxidant enzyme cytosolic superoxide dismutase and the structural protein filamin confers protection against the adult worm stage.Infection and immunity · 2004Article
- Cross-reactivity of Schistosoma mansoni cytosolic superoxide dismutase, a protective vaccine candidate, with host superoxide dismutase and identification of parasite-specific B epitopes.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Antioxidants may play an important role in immune evasion by schistosome parasites. Previous studies have focused on the roles of superoxide dismutase and glutathione S-transferase. In the present study, glutathione peroxidase (GPX) activity was measured in different fractions of worm extracts from several developmental stages of Schistosoma mansoni. The enzyme activity was shown to be developmentally regulated, with higher specific activities being found in the tegument-enriched Nonidet P-40 extract of adult worms (the stage least susceptible to immune killing) than in the larval stages (which are most susceptible to immune elimination). In all extracts tested, the activity against cumene hydroperoxide, even when glutathione S-transferase activity was removed, was higher than that for hydrogen peroxide. The expression of GPX cDNA in pGEX-2T by bacteria produced a 50-kDa fusion protein and a 32-kDa truncated protein. The latter was due to termination at the internal UGA codon that codes for selenocysteine. GPX activity was detected in the recombinantly produced GPX but not with Sj26-glutathione S-transferase from the vector. Mutating the TGA codon to TGT produced a full-length product, GPXm (19 kDa), that was used to produce 19 monoclonal antibodies. Anti-GPXm monoclonal antibodies recognized a 19-kDa molecule in adult-worm extract which, upon removal by immunoprecipitation, resulted in the loss of over 90% of the GPX activity, suggesting that a single form of GPX exists in the schistosome.
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