ArticleThe Journal of cell biology1996
Two independent domains of hDlg are sufficient for subcellular targeting: the PDZ1-2 conformational unit and an alternatively spliced domain.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 119 citations in OpenAlex.
- The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration.Science advances · 2022Article
- Structures of the L27 Domain of Disc Large Homologue 1 Protein Illustrate a Self-Assembly Module.Biochemistry · 2018Article
- PDZ Domain Recognition: Insight from Human Tax-Interacting Protein 1 (TIP-1) Interaction with Target Proteins.Biology · 2015Review
- Selective phosphorylation of the Dlg1AB variant is critical for TCR-induced p38 activation and induction of proinflammatory cytokines in CD8+ T cells.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- The human adenovirus E4-ORF1 protein subverts discs large 1 to mediate membrane recruitment and dysregulation of phosphatidylinositol 3-kinase.PLoS pathogens · 2014Article
- Dishevelled binds the Discs large 'Hook' domain to activate GukHolder-dependent spindle positioning in Drosophila.PloS one · 2014Article
- Interactome analysis reveals ezrin can adopt multiple conformational states.The Journal of biological chemistry · 2013Article
- Structural insights into a wildtype domain of the oncoprotein E6 and its interaction with a PDZ domain.PloS one · 2013Article
- The scaffolding protein synapse-associated protein 97 is required for enhanced signaling through isotype-switched IgG memory B cell receptors.Science signaling · 2012Article
- The invasive capacity of HPV transformed cells requires the hDlg-dependent enhancement of SGEF/RhoG activity.PLoS pathogens · 2012Article
- Article
- Polarity protein complex Scribble/Lgl/Dlg and epithelial cell barriers.Advances in experimental medicine and biology · 2012Article
- Essential function of protein 4.1G in targeting of membrane protein palmitoylated 6 into Schmidt-Lanterman incisures in myelinated nerves.Molecular and cellular biology · 2012Article
- The MEK2-binding tumor suppressor hDlg is recruited by E-cadherin to the midbody ring.BMC cell biology · 2011Article
- Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.The EMBO journal · 2010Article
- The unique-5 and -6 motifs of ZO-1 regulate tight junction strand localization and scaffolding properties.Molecular biology of the cell · 2007Article
- Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies.Infection and immunity · 2006Article
- Oncogenic function for the Dlg1 mammalian homolog of the Drosophila discs-large tumor suppressor.The EMBO journal · 2006Article
- Association of an A-kinase-anchoring protein signaling scaffold with cadherin adhesion molecules in neurons and epithelial cells.Molecular biology of the cell · 2005Article
- Discs large (Dlg1) complexes in lymphocyte activation.The Journal of cell biology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
hDlg, a human homologue of the Drosophila Dig tumor suppressor, contains two binding sites for protein 4.1, one within a domain containing three PSD-95/Dlg/ZO-1 (PDZ) repeats and another within the alternatively spliced I3 domain. Here, we further define the PDZ-protein 4.1 interaction in vitro and show the functional role of both 4.1 binding sites in situ. A single protease-resistant structure formed by the entirety of both PDZ repeats 1 and 2 (PDZ1-2) contains the protein 4.1-binding site. Both this PDZ1-2 site and the I3 domain associate with a 30-kD NH2-terminal domain of protein 4.1 that is conserved in ezrin/radixin/moesin (ERM) proteins. We show that both protein 4.1 and the ezrin ERM protein interact with the murine form of hDlg in a coprecipitating immune complex. In permeabilized cells and tissues, either the PDZ1-2 domain or the I3 domain alone are sufficient for proper subcellular targeting of exogenous hDlg. In situ, PDZ1-2-mediated targeting involves interactions with both 4.1/ERM proteins and proteins containing the COOH-terminal T/SXV motif. I3-mediated targeting depends exclusively on interactions with 4.1/ERM proteins. Our data elucidates the multivalent nature of membrane-associated guanylate kinase homologue (MAGUK) targeting, thus beginning to define those protein interactions that are critical in MAGUK function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.