ArticleThe Biochemical journal1996
The lysosomal Ca2+ pool in MDCK cells can be released by ins(1,4,5)P3-dependent hormones or thapsigargin but does not activate store-operated Ca2+ entry.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Flower dependent trafficking of lamellar bodies facilitates maturation of the epidermal barrier.Nature communications · 2025Article
- Lysosomal agents inhibit store-operated CaJournal of cell science · 2021Article
- The GIMAP Family Proteins: An Incomplete Puzzle.Frontiers in immunology · 2021Review
- TFEB-mediated increase in peripheral lysosomes regulates store-operated calcium entry.Scientific reports · 2017Article
- Connecting CaMessenger (Los Angeles, Calif. : Print) · 2016Article
- Article
- A cation counterflux supports lysosomal acidification.The Journal of cell biology · 2010Article
- Ca2+ accumulation into acidic organelles mediated by Ca2+- and vacuolar H+-ATPases in human platelets.The Biochemical journal · 2005Article
- Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 is required for lysosome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Endolysosomal proteolysis and its regulation.The Biochemical journal · 2002Review
- The role of intraorganellar Ca(2+) in late endosome-lysosome heterotypic fusion and in the reformation of lysosomes from hybrid organelles.The Journal of cell biology · 2000Article
- Transduction of basolateral-to-apical signals across epithelial cells: ligand-stimulated transcytosis of the polymeric immunoglobulin receptor requires two signals.Molecular biology of the cell · 1999Article
- Role of tyrosine phosphorylation in ligand-induced regulation of transcytosis of the polymeric Ig receptor.Molecular biology of the cell · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In several cell types, Ca2+ release from intracellular Ca2+ stores by Ins(1,4,5)P3 elicits Ca2+ influx from the extracellular space into the cytoplasm, termed store-operated Ca2+ entry (SOCE). In MDCK cells, the Ins(1,4,5)P3-sensitive Ca2+ store giving rise to SOCE essentially overlaps with the thapsigargin (TG)-sensitive store. Recent evidence suggests that in MDCK cells lysosomes form a Ca2+ pool that is functionally coupled with the Ins(1,4,5)P3-sensitive Ca2+ store: Ca2+ can be selectively released from lysosomes by glycyl-L-phenylalanine naphthylamide, an agent inducing lysosomal swelling with subsequent and reversible permeabilization of the vesicular membranes. This compartment is also depleted by Ins(1,4,5)P3-dependent agonists or TG, indicating that it is part of a larger, Ins(1,4,5)P3-sensitive Ca2+ pool. Here we show that whereas SOCE is triggered by Ca2+ release from the entire Ins(1,4,5)P3-sensitive Ca2+ pool, selective Ca2+ release from lysosomes alone is unable to trigger SOCE. This finding is consistent with measurements of the store-operated cation current, a direct parameter for store-operated Ca2+ and Na+ entry into MDCK cells. Hence it is proposed that the Ins(1,4,5)P3-sensitive Ca2+ pool is composed of different intracellular compartments that do not uniformly stimulate Ca2+ entry into the cell.
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