ArticleThe Journal of experimental medicine1996
Proteasome activity is required for the stage-specific transformation of a protozoan parasite.
Article in The Journal of experimental medicine, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 105 citations in OpenAlex.
- Article
- Revisiting cysteine protease function inMicrobiology spectrum · 2026Article
- TrypPROTACs Unlocking New Therapeutic Strategies for Chagas Disease.Pharmaceuticals (Basel, Switzerland) · 2025Review
- The proteasome as a drug target for treatment of parasitic diseases.Advances in parasitology · 2024Review
- Targeted protein degradation might present a novel therapeutic approach in the fight against African trypanosomiasis.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · 2023Article
- Article
- Ubiquitination and the Proteasome as Drug Targets in Trypanosomatid Diseases.Frontiers in chemistry · 2020Review
- The proteasome as a target: How not tidying up can have toxic consequences for parasitic protozoa.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis inFrontiers in cellular and infection microbiology · 2019Review
- Emerging therapeutic targets for treatment of leishmaniasis.Expert opinion on therapeutic targets · 2018Review
- Ubiquitin Proteasome pathway proteins as potential drug targets in parasite Trypanosoma cruzi.Scientific reports · 2018Article
- Archaea Symbiont ofFrontiers in cellular and infection microbiology · 2018Article
- The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis.PLoS neglected tropical diseases · 2017Article
- Article
- Article
- Role of the Ubiquitin-Proteasome Systems in the Biology and Virulence of Protozoan Parasites.BioMed research international · 2015Review
- The Trypanosoma cruzi flagellum is discarded via asymmetric cell division following invasion and provides early targets for protective CD8⁺ T cells.Cell host & microbe · 2014Article
- Investigation on the 19S ATPase proteasome subunits (Rpt1-6) conservation and their differential gene expression in Schistosoma mansoni.Parasitology research · 2013Article
- Proteomic analysis of Trypanosoma cruzi epimastigotes subjected to heat shock.Journal of biomedicine & biotechnology · 2012Article
- Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.PloS one · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A prominent feature of the life cycle of intracellular parasites is the profound morphological changes they undergo during development in the vertebrate and invertebrate hosts. In eukaryotic cells, most cytoplasmic proteins are degraded in proteasomes. Here, we show that the transformation in axenic medium of trypomastigotes of Trypanosoma cruzi into amastigote-like organisms, and the intracellular development of the parasite from amastigotes into trypomastigotes, are prevented by lactacystin, or by a peptide aldehyde that inhibits proteasome function. Clasto-lactacystin, an inactive analogue of lactacystin, and cell-permeant peptide aldehyde inhibitors of T. cruzi cysteine proteinases have no effect. We have also identified the 20S proteasomes from T. cruzi as a target of lactacystin in vivo. Our results document the essential role of proteasomes in the stage-specific transformation of a protozoan.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.