ArticleThe EMBO journal1996
Loss of growth polarity and mislocalization of septa in a Neurospora mutant altered in the regulatory subunit of cAMP-dependent protein kinase.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 132 citations in OpenAlex.
- The PKR regulatory subunit of protein kinase A (PKA) is involved in the regulation of growth, sexual and asexual development, and pathogenesis in Fusarium graminearum.Molecular plant pathology · 2018Article
- Identification and characterization of the glucose dual-affinity transport system inBiotechnology for biofuels · 2017Article
- Regulatory roles of phosphorylation in model and pathogenic fungi.Medical mycology · 2016Review
- The Regulatory Subunit of Protein Kinase A (Bcy1) inFrontiers in microbiology · 2016Article
- Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose.BMC microbiology · 2015Article
- Isolation and Characterization of the PKAr Gene From a Plant Pathogen, Curvularia lunata.Indian journal of microbiology · 2014Article
- Protein kinase A regulatory subunit isoforms regulate growth and differentiation in Mucor circinelloides: essential role of PKAR4.Eukaryotic cell · 2012Article
- RIC8 is a guanine-nucleotide exchange factor for Galpha subunits that regulates growth and development in Neurospora crassa.Genetics · 2011Article
- A Screen for Germination Mutants in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2011Article
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- The BEM46-like protein appears to be essential for hyphal development upon ascospore germination in Neurospora crassa and is targeted to the endoplasmic reticulum.Current genetics · 2009Article
- Protein kinase A regulates growth, sporulation, and pigment formation in Aspergillus fumigatus.Applied and environmental microbiology · 2008Article
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- Protein kinase A and casein kinases mediate sequential phosphorylation events in the circadian negative feedback loop.Genes & development · 2007Article
- Localization of RHO-4 indicates differential regulation of conidial versus vegetative septation in the filamentous fungus Neurospora crassa.Eukaryotic cell · 2007Article
- The STE20/germinal center kinase POD6 interacts with the NDR kinase COT1 and is involved in polar tip extension in Neurospora crassa.Molecular biology of the cell · 2006Article
- Mucor rouxii ultrastructure: cyclic AMP and actin cytoskeleton.Protoplasma · 2006Article
- Polarisome meets spitzenkörper: microscopy, genetics, and genomics converge.Eukaryotic cell · 2005Review
- Severe impairment of growth and differentiation in a Neurospora crassa mutant lacking all heterotrimeric G alpha proteins.Genetics · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In filamentous fungi, growth polarity (i.e. hyphal extension) and formation of septa require polarized deposition of new cell wall material. To explore this process, we analyzed a conditional Neurospora crassa mutant, mcb, which showed a complete loss of growth polarity when incubated at the restrictive temperature. Cloning and DNA sequence analysis of the mcb gene revealed that it encodes a regulatory subunit of cAMP-dependent protein kinase (PKA). Unexpectedly, the mcb mutant still formed septa when grown at the restrictive temperature, indicating that polarized deposition of wall material during septation is a process that is, at least in part, independent of polarized deposition during hyphal tip extension. However, septa formed in the mcb mutant growing at the restrictive temperature are mislocalized. Both polarized growth and septation are actin-dependent processes, and a concentration of actin patches is observed at growing hyphal tips and sites where septa are being formed. In the mcb mutant growing at the restrictive temperature, actin patches are uniformly distributed over the cell cortex; however, actin patches are still concentrated at sites of septation. Our results suggest that the PKA pathway regulates hyphal growth polarity, possibly through organizing actin patches at the cell cortex.
Indexed as
Identifiers
8918454PMC452324W54333096What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.