ArticleProceedings of the National Academy of Sciences of the United States of America1996
Rapid chemical kinetic techniques for investigations of neurotransmitter receptors expressed in Xenopus oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 17 citations in OpenAlex.
- The "www" ofMembranes · 2022Review
- Sub-millisecond ligand probing of cell receptors with multiple solution exchange.Nature protocols · 2013Article
- A chemical waveform synthesizer.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Practical limits on the maximal speed of solution exchange for patch clamp experiments.Biophysical journal · 1999Article
- The effects of level of expression of a jellyfish Shaker potassium channel: a positive potassium feedback mechanism.The Journal of physiology · 1999Article
- Thermal imaging of receptor-activated heat production in single cells.Biophysical journal · 1998Article
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7 authors at 2 institutions in 2 countries.
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Abstract
Xenopus laevis oocytes have been used extensively during the past decade to express and study neurotransmitter receptors of various origins and subunit composition and also to express and study receptors altered by site-specific mutations. Interpretations of the effects of structural differences on receptor mechanisms were, however, hampered by a lack of rapid chemical reaction techniques suitable for use with oocytes. Here we describe flow and photolysis techniques, with 2-ms and 100-microseconds time resolution, respectively, for studying neurotransmitter receptors in giant (approximately 20-microns diameter) patches of oocyte membranes, using muscle and neuronal acetylcholine receptors as examples. With these techniques, we find that the muscle receptor in BC3H1 cells and the same receptor expressed in oocytes have comparable kinetic properties. This finding is in contrast to previous studies and raises questions regarding the interpretations of the many studies of receptors expressed in oocytes in which an insufficient time resolution was available. The results obtained indicate that the rapid reaction techniques described here, in conjunction with the oocyte expression system, will be useful in answering many outstanding questions regarding the structure and function of diverse neurotransmitter receptors.
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