ArticleMolecular & general genetics : MGG1996
The 3' untranslated regions of chloroplast genes in Chlamydomonas reinhardtii do not serve as efficient transcriptional terminators.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 49 citations in OpenAlex.
- Evolution of mitochondrial RNA editing sites and stop codon-lacking transcripts in angiosperms.Communications biology · 2025Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- The effect of different 3' untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco.Plant molecular biology · 2011Article
- A novel class of heat-responsive small RNAs derived from the chloroplast genome of Chinese cabbage (Brassica rapa).BMC genomics · 2011Article
- Function of chloroplast RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2011Review
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- The nucleus-encoded factor MCD4 participates in degradation of nonfunctional 3' UTR sequences generated by cleavage of pre-mRNA in Chlamydomonas chloroplasts.Molecular genetics and genomics : MGG · 2007Article
- Antisense transcript and RNA processing alterations suppress instability of polyadenylated mRNA in chlamydomonas chloroplasts.The Plant cell · 2004Article
- Short dispersed repeats in the Chlamydomonas chloroplast genome are collocated with sites for mRNA 3' end formation.Current genetics · 2004Article
- Chloroplast RNA processing and stability.Photosynthesis research · 2004Article
- The stem-loop region of the tobacco psbA 5'UTR is an important determinant of mRNA stability and translation efficiency.Molecular genetics and genomics : MGG · 2003Article
- The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats.The Plant cell · 2002Article
- Evidence for in vivo modulation of chloroplast RNA stability by 3'-UTR homopolymeric tails in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A role for initiation codon context in chloroplast translation.The Plant cell · 2001Article
- The sequence and secondary structure of the 3'-UTR affect 3'-end maturation, RNA accumulation, and translation in tobacco chloroplasts.Plant molecular biology · 2000Article
- Characterization of the E.coli poly(A) polymerase: nucleotide specificity, RNA-binding affinities and RNA structure dependence.Nucleic acids research · 2000Article
- Altering the 3 UTR endonucleolytic cleavage site of a Chlamydomonas chloroplast mRNA affects 3-end maturation in vitro but not in vivo.Plant molecular biology · 1999Article
- 3'-Processed mRNA is preferentially translated in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 1998Article
- The chloroplast atpA gene cluster in Chlamydomonas reinhardtii. Functional analysis of a polycistronic transcription unit.Plant physiology · 1998Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
A general characteristic of the 3' untranslated regions of plastid mRNAs is an inverted repeat sequence that can fold into a stem-loop structure. These stem-loops are superficially similar to structures involved in prokaryotic transcription termination, but were found instead to serve as RNA 3' end processing signals in spinach chloroplasts, and in the atpB mRNA of Chlamydomonas reinhardtii chloroplasts. In order to carry out a broad study of the efficiency of the untranslated sequences at the 3' ends of chloroplast genes in Chlamydomonas to function as transcription terminators, we performed in vivo run-on transcription experiments using Chlamydomonas chloroplast transformants in which different 3' ends were inserted into the chloroplast genome between a petD promoter and a reporter gene. The results showed that none of the 3' ends that were tested, in either sense or antisense orientation, prevented readthrough transcription, and thus were not highly efficient transcription terminators. Therefore, we suggest that most or all of the 3' ends of mature mRNAs in Chlamydomonas chloroplasts are formed by 3' end processing of longer precursors.
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