ArticleClinical and diagnostic laboratory immunology1996
Detection of Toxoplasma gondii tachyzoites and bradyzoites in blood, urine, and brains of infected mice.
Article in Clinical and diagnostic laboratory immunology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Live-attenuatedVirulence · 2026Article
- Viability ofVeterinary world · 2022Article
- Detection of toxoplasmic encephalitis in HIV positive patients in urine with hydrogel nanoparticles.PLoS neglected tropical diseases · 2021Article
- Development of Nano-ELISA Method for Serological Diagnosis of Toxoplasmosis in Mice.Archives of Razi Institute · 2021Article
- Detection of Plasmodium knowlesi DNA in the urine and faeces of a Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection.Malaria journal · 2014Article
- Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia.Veterinary research forum : an international quarterly journal · 2013Article
- Improving urine sample efficacy as a convenient alternative for invasive samples in molecular diagnosis of toxoplasmosis.Iranian journal of parasitology · 2013Article
- Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2012Article
- Sequential recognition of antigenic markers of Toxoplasma gondii tachyzoite by pooled sera of mice with experimental toxoplasmosis.Parasitology research · 2011Article
- GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii-specific immunoglobulin G in humans with acute toxoplasmosis.Clinical and vaccine immunology : CVI · 2009Article
- Development of a PCR-enzyme immunoassay oligoprobe detection method for Toxoplasma gondii oocysts, incorporating PCR controls.Applied and environmental microbiology · 2003Article
- Acute and chronic phases of Toxoplasma gondii infection in mice modulate the host immune responses.Infection and immunity · 1998Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different techniques for identifying Toxoplasma gondii were compared. PCR was used to amplify part of the major surface antigen P30 gene of T. gondii. Amplified-DNA detection with the DNA enzyme immunoassay (PCR-DEIA) was more sensitive than ethidium bromide staining after agarose gel electrophoresis and as sensitive as nested PCR. PCR-DEIA, using common enzyme-linked immunosorbent assay (ELISA) methods, avoids agarose gel electrophoresis for the identification of amplified products. T. gondii can also be detected with equal sensitivity in infected fibroblasts, but only after at least 8 days of cell culture. PCR-DEIA is thus recommended because of its sensitivity and convenience for detecting early parasitemia in the surveillance of toxoplasmosis among pregnant women and immunocompromised hosts. The courses of infection in mice infected with two strains of T. gondii were compared. Tachyzoites of the virulent strain T. gondii RH, killing the host in 4 days, were identified in urine specimens and blood samples of mice 24 to 94 h after inoculation but not in brains, but no antibodies were detected. After intraperitoneal inoculation with cysts of the low-level virulence Beverley strain of T. gondii, parasites were identified in blood samples 4 days later and up to 17 days (but not in urine specimens) and in the brain from day 6 through day 525. By ELISA, high antibody titers were found from day 11 to day 525, with parasitemia preceding the appearance of antibodies. The usefulness of PCR-DEIA tests in conjunction with the search for circulating antibodies for the early diagnosis of toxoplasmosis in humans is discussed.
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