ArticlePlant molecular biology1996
Transcription of CABII is regulated by the biological clock in Chlamydomonas reinhardtii.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Identification of novel clock-controlled genes by cDNA macroarray analysis in Chlamydomonas reinhardtii.Plant molecular biology · 2005Article
- The circadian clock in Chlamydomonas reinhardtii. What is it for? What is it similar to?Plant physiology · 2005Review
- Paths toward algal genomics.Plant physiology · 2005Review
- Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene.Plant molecular biology · 2004Article
- The CPH1 gene of Chlamydomonas reinhardtii encodes two forms of cryptochrome whose levels are controlled by light-induced proteolysis.Plant physiology · 2004Article
- A genome's-eye view of the light-harvesting polypeptides of Chlamydomonas reinhardtii.Current genetics · 2004Review
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- Assessing the relative importance of light and the circadian clock in controlling chloroplast translation in Chlamydomonas reinhardtii.Photosynthesis research · 2002Article
- Identification of short promoter regions involved in the transcriptional expression of the nitrate reductase gene in Chlamydomonas reinhardtii.Plant molecular biology · 2001Article
- Requirement for cytoplasmic protein synthesis during circadian peaks of transcription of chloroplast-encoded genes in Chlamydomonas.Plant molecular biology · 2000Article
- The Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase is encoded by a light-regulated gene in Chlamydomonas reinhardtii.Plant molecular biology · 1998Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The small gene family encoding the chlorophyll a/b-binding proteins of photosystem II (CABII or lhcb) is known to exhibit circadian rhythms of mRNA abundance in Chlamydomonas reinhardtii. In this study we investigated the role of transcription in the phenomenon. We used as reporters Chlamydomonas genes that encode nitrate reductase (NITI) and arylsulfatase (ARS2) transcriptionally fused to sequences upstream of one of the CABII genes (called CABII-1). We found that both reporters exhibited the same circadian rhythm of mRNA abundance in phase, period, and amplitude as does the endogenous CABII-1 gene. We also evaluated the efficacy of arylsulfatase enzymatic activity as a reporter and found that its half-life is too long to make it a useful reporter of rhythmic transcription during a circadian or diurnal cycle. The amount of mRNA synthesis from the CABII-1 gene was examined by in vivo labeling experiments and a circadian rhythm in transcription rate was demonstrated. In vivo labeling also revealed a circadian rhythm of mRNA synthesis for the CABII gene family as a whole. The results from the transcriptional reporter assays together with the in vivo labeling experiments strongly support the conclusion that the biological clock regulates the transcriptional activity of the CABII-I gene, and moreover that regulation at the transcriptional level is the predominant mode by which the clock regulates this gene.
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