ArticlePlant molecular biology1996
Regulation of an embryogenic carrot gene (DC 2.15) and identification of its active promoter sites.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.Plants (Basel, Switzerland) · 2022Review
- Isolation and characterization of genes associated to cotton somatic embryogenesis by suppression subtractive hybridization and macroarray.Plant molecular biology · 2006Article
- A cell wall protein down-regulated by auxin suppresses cell expansion in Daucus carota (L.).Plant molecular biology · 2002Article
- Partial characterization of genes whose transcripts accumulate preferentially in cell clusters at the earliest stage of carrot somatic embryogenesis.Plant molecular biology · 2001Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
According to previous studies the expression of the gene DC 2.15 is induced in cultured carrot cells after a transfer to an auxin-free medium, where somatic embryo development occurs. This embryogenic gene encodes a prolinerich protein, which resembles proteins involved in auxin-controlled developmental processes. To understand the mechanism underlying the regulation of DC 2.15, an experimental approach has been employed which allows the direct identification of the DC 2.15 promoter structure by applying PCR techniques. We demonstrate the presence of five distinct promoter sequences highly similar in structure, but slightly different in a common region of about 15 nucleotides, which contain the binding site for the GATA factor originally found in the human HOX gene. Activity of each promoter structure was assessed in developing somatic embryos containing the specific sequence fused to the beta-glucuronidase (GUS) reporter gene. For two of the five promoter structures a drastic increase in activity was registered during the torpedo stage while the remaining three were inactive throughout the stages of somatic embryogenesis.
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Registered trials
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