ArticleBiophysical journal1996
Electrical manipulation of glycan-phosphatidyl inositol-tethered proteins in planar supported bilayers.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 118 citations in OpenAlex.
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- Rapid Enrichment of a Native Multipass Transmembrane Protein via Cell Membrane Electrophoresis through Buffer pH and Ionic Strength Adjustment.Journal of the American Chemical Society · 2024Article
- Self-Quenching Behavior of a Fluorescent Probe Incorporated within Lipid Membranes Explored Using Electrophoresis and Fluorescence Lifetime Imaging Microscopy.The journal of physical chemistry. B · 2023Article
- Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers.The European physical journal. E, Soft matter · 2016Article
- Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer.ChemistryOpen · 2016Article
- Crowding-Induced Mixing Behavior of Lipid Bilayers: Examination of Mixing Energy, Phase, Packing Geometry, and Reversibility.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Covalent Ras Dimerization on Membrane Surfaces through Photosensitized Oxidation.Journal of the American Chemical Society · 2016Article
- Spatial organization of EphA2 at the cell-cell interface modulates trans-endocytosis of ephrinA1.Biophysical journal · 2014Article
- Electrophoretic measurements of lipid charges in supported bilayers.Analytical chemistry · 2013Article
- Nanofabrication for the analysis and manipulation of membranes.Annals of biomedical engineering · 2012Review
- EphA2 receptor activation by monomeric Ephrin-A1 on supported membranes.Biophysical journal · 2011Article
- Protein separation by electrophoretic-electroosmotic focusing on supported lipid bilayers.Analytical chemistry · 2011Article
- Supported bilayer electrophoresis under controlled buffer conditions.Analytical chemistry · 2011Article
- Article
- Biomimetic silica microspheres in biosensing.Molecules (Basel, Switzerland) · 2010Review
- Biology under construction: in vitro reconstitution of cellular function.Nature reviews. Molecular cell biology · 2009Review
- Biophysical effects of electric fields on membrane water interfaces: a mini review.European biophysics journal : EBJ · 2007Review
- Separation of membrane-bound compounds by solid-supported bilayer electrophoresis.Journal of the American Chemical Society · 2007Article
- Suppressing Brownian motion of individual biomolecules in solution.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Controlling two-dimensional tethered vesicle motion using an electric field: interplay of electrophoresis and electro-osmosis.Langmuir : the ACS journal of surfaces and colloids · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electric fields have been used to manipulate and concentrate glycan-phosphatidyl inositol (GPI)-tethered proteins in planar supported bilayers. Naturally GPI-linked CD48, along with engineered forms of I-Ek and B7-2, in which their transmembrane domains have been genetically replaced with the GPI linkage, were studied. The proteins were labeled with fluorescently tagged antibodies, allowing the electric field-induced behavior to be followed by epifluorescence microscopy. All three protein complexes were observed to migrate toward the cathode with the B7-2 and CD48, each tethered to the membrane by a single GPI linker, moving significantly faster than the I-Ek, which has two GPI linkers. Patterns scratched into the membrane function as barriers to lateral diffusion and were used to isolate the proteins into highly concentrated corrals. All field-induced concentration profiles were completely reversible, indicating that the supported bilayer provides a stable, fluid environment in which GPI-tethered proteins can be manipulated. The ability to electrically control the spatial distribution of membrane-tethered proteins provides new opportunities for the study of biological membranes and the development of membrane-based devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.