ArticleBiophysical journal1996
Atomic force microscopy study of the secretory granule lumen.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- A novel cysteine-rich adaptor protein is required for mucin packaging and secretory granule stability in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Probing single molecule mechanical interactions of syntaxin 1A with native synaptobrevin 2 residing on a secretory vesicle.Cell calcium · 2022Article
- The Physiological and Pathological Implications of the Formation of Hydrogels, with a Specific Focus on Amyloid Polypeptides.Biomolecules · 2017Review
- The hydrogel nature of mammalian cytoplasm contributes to osmosensing and extracellular pH sensing.Biophysical journal · 2009Article
- Understanding apoptotic signaling pathways in cytosine deaminase-uracil phosphoribosyl transferase-mediated suicide gene therapy in vitro.Molecular and cellular biochemistry · 2009Article
- The trans-Golgi proteins SCLIP and SCG10 interact with chromogranin A to regulate neuroendocrine secretion.Biochemistry · 2008Article
- Probing nanomechanical properties from biomolecules to living cells.Pflugers Archiv : European journal of physiology · 2008Review
- Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy.Croatica chemica acta. Arhiv za kemiju · 2008Article
- A mechanical spike accompanies the action potential in Mammalian nerve terminals.Biophysical journal · 2007Article
- The force-driven conformations of heparin studied with single molecule force microscopy.Biophysical journal · 2003Article
- Control of neurotransmitter release by an internal gel matrix in synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mechanical forces impeding exocytotic surfactant release revealed by optical tweezers.Biophysical journal · 2003Article
- Dynamics of fusion pores connecting membranes of different tensions.Biophysical journal · 2000Article
- Mechanism of peptide-induced mast cell degranulation. Translocation and patch-clamp studies.The Journal of general physiology · 1998Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- Regulation of exocytotic fusion by cell inflation.Biophysical journal · 1998Article
- Gi regulation of secretory vesicle swelling examined by atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Kinetics of release of serotonin from isolated secretory granules. II. Ion exchange determines the diffusivity of serotonin.Biophysical journal · 1997Article
- Cellular and molecular mechanics by atomic force microscopy: capturing the exocytotic fusion pore in vivo?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used an atomic force microscope to study the mechanical properties of the matrix found in the lumen of secretory granules isolated from mast cells. The matrices were insoluble and had an average height of 474 +/- 197 nm. The volume of these matrices increased reversibly about tenfold by decreasing the valency of the bathing external cation (La3+ < Ca2+ < Na+). The elastic (Young's) modulus was found to decrease by about 100-fold (4.3 MPa in La3+ to 37 kPa in Na+) upon a tenfold increase in the matrix volume. A swollen granule matrix had an elastic modulus similar to that of gelatin in water. The elastic modulus was inversely related to the change in the volume of the matrix, following a relationship similar to that predicted for the elasticity of weakly cross-linked polymers. Our results show that the matrix of these secretory granules have the mechanical properties of weak ion exchange resins, lending strong support to an ion exchange mechanism for the storage and release of cationic secretory products.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.