ArticleThe Biochemical journal1996
Vitamin E potentiates arachidonate release and phospholipase A2 activity in rat heart myoblastic cells.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 47 citations in OpenAlex.
- Replacement of Vitamin E by an Extract from an Olive Oil By-Product, Rich in Hydroxytyrosol, in Broiler Diets: Effects on Growth Performance and Breast Meat Quality.Antioxidants (Basel, Switzerland) · 2023Article
- The Impact of Vitamin E and Other Fat-Soluble Vitamins on Alzheimer´s Disease.International journal of molecular sciences · 2016Review
- The role of vitamin e in human health and some diseases.Sultan Qaboos University medical journal · 2014Review
- Oxidative damage and antioxidative therapy in systemic sclerosis.Mediators of inflammation · 2014Article
- Low molecular weight phospholipases A2 in mammalian brain and neural cells: roles in functions and dysfunctions.Molecular neurobiology · 2010Review
- Nonenzymatic lipid peroxidation reprograms gene expression and activates defense markers in Arabidopsis tocopherol-deficient mutants.The Plant cell · 2006Article
- Characterization of tocopherol cyclases from higher plants and cyanobacteria. Evolutionary implications for tocopherol synthesis and function.Plant physiology · 2003Article
- Alpha-tocopherol modulates the low density lipoprotein receptor of human HepG2 cells.Nutrition journal · 2003Article
- Regulation of cytosolic phospholipase A2, cyclooxygenase-1 and -2 expression by PMA, TNFalpha, LPS and M-CSF in human monocytes and macrophages.Molecular and cellular biochemistry · 2003Article
- Interaction of alpha-tocopherol with model human high-density lipoproteins.Biophysical journal · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic phospholipase A2 (cPLA2) selectively catalyses the release of arachidonic acid from the sn-2 position of glycero-phospholipids to produce prostaglandins and leukotrienes. In this study, vitamin E enrichment of rat heart myoblastic H9c2 cells caused an increase in the release of arachidonate during ionophore (A23187) stimulation. PLA2 activity in the cytosolic fraction was also enhanced but enzyme activity in the particulate fraction was not affected by this treatment. Immunoblotting analysis with a polyclonal anti-cPLA2 antibody showed an increased level of the enzyme in vitamin E-treated cells. Direct incorporation of vitamin E into lipid vesicles in the assay mixture resulted in modulation of enzyme activity in a biphasic manner. Pretreatment of cells with phorbol 12-myristate 13-acetate, a known activator of protein kinase C, synergistically potentiated the ionophore-induced arachidonate release in both the control and vitamin E-treated cells. However, vitamin E treatment by itself did not affect the protein kinase C activity, indicating that the vitamin E-induced activation of cPLA2 was independent of the protein kinase C cascade. Collectively, these results suggest that vitamin E potentiates arachidonate release through the direct and/or indirect modulation of cPLA2 activity.
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