ArticleThe Journal of physiology1996
Role of Rho proteins in carbachol-induced contractions in intact and permeabilized guinea-pig intestinal smooth muscle.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 110 citations in OpenAlex.
- Characterization of Cecal Smooth Muscle Contraction in Laying Hens.Veterinary sciences · 2021Article
- Light-Mediated Inhibition of Colonic Smooth Muscle Constriction and Colonic MotilityFrontiers in physiology · 2021Article
- Calcium Sensitization Mechanisms in Gastrointestinal Smooth Muscles.Journal of neurogastroenterology and motility · 2016Review
- Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2014Article
- Soluble guanylate cyclase generation of cGMP regulates migration of MGE neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Direct binding and regulation of RhoA protein by cyclic GMP-dependent protein kinase Iα.The Journal of biological chemistry · 2012Article
- 3',4'-Dihydroxyflavonol reduces vascular contraction through Ca²⁺ desensitization in permeabilized rat mesenteric artery.Naunyn-Schmiedeberg's archives of pharmacology · 2012Article
- Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-alpha.Lasers in medical science · 2010Article
- The effects of the small GTPase RhoA on the muscarinic contraction of airway smooth muscle result from its role in regulating actin polymerization.American journal of physiology. Cell physiology · 2010Article
- Androgens induce nongenomic stimulation of colonic contractile activity through induction of calcium sensitization and phosphorylation of LC20 and CPI-17.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Calcium sensitization induced by sodium fluoride in permeabilized rat mesenteric arteries.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010Article
- RhoA/Rho-kinase: pathophysiologic and therapeutic implications in gastrointestinal smooth muscle tone and relaxation.Gastroenterology · 2010Review
- RhoA/Rho-kinase signaling: a therapeutic target in pulmonary hypertension.Vascular health and risk management · 2009Review
- Involvement of Rho kinase and protein kinase C in carbachol-induced calcium sensitization in beta-escin skinned rat and guinea-pig bladders.British journal of pharmacology · 2006Article
- Involvement of RhoA-mediated Ca2+ sensitization in antigen-induced bronchial smooth muscle hyperresponsiveness in mice.Respiratory research · 2005Article
- Mechanisms involved in carbachol-induced Ca(2+) sensitization of contractile elements in rat proximal and distal colon.British journal of pharmacology · 2004Article
- Modulation of RhoA-Rho kinase-mediated Ca2+ sensitization of rabbit myometrium during pregnancy - role of Rnd3.The Journal of physiology · 2003Article
- The role of RhoA and Rho-associated kinase in vascular smooth muscle contraction.Current hypertension reports · 2003Review
- Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle.The Biochemical journal · 2003Article
- Augmented acetylcholine-induced translocation of RhoA in bronchial smooth muscle from antigen-induced airway hyperresponsive rats.British journal of pharmacology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The aim of this study was to determine whether the low molecular mass GTPase RhoA or related proteins are involved in carbachol- and high-K(+)-induced contractions in intact intestinal smooth muscle as well as the carbachol-induced increase in Ca2+ sensitivity of the myofilaments in permeabilized preparations. 2. The carbachol-induced increase in the Ca2+ sensitivity of force production in beta-escin-permeabilized intestinal smooth muscle was enhanced in preparations that were loaded with the constitutively active mutant of RhoA, Val14RhoA, and was inhibited by exoenzyme C3 from Clostridium botulinum, which ADP-ribosylates and inactivates small GTPases of the Rho family. The effect of C3 on Ca2+ sensitivity in the absence of the agonist was negligible, while the maximal Ca(2+)-activated force was inhibited by about 20%. 3. Inhibition of carbachol-induced force was associated with an increase in ADP-ribosylation of a protein band with a molecular mass of approximately 22 kDa, corresponding to Rho, and was partially reversed in the presence of Ile41RhoA, which is not a substrate for C3. Val14RhoA did not restore carbachol-induced Ca2+ sensitization in C3-treated smooth muscle. 4. In intact intestinal smooth muscle, toxin B from Clostridium difficile, which monoglucosylates members of the Rho family, inhibited high-K(+)-induced contractions and the initial phasic response to carbachol by about 30%. The delayed contractile response to carbachol was completely inhibited. 5. In smooth muscle preparations that were permeabilized with beta-escin after treatment with toxin B, carbachol-and GTP gamma S-induced Ca2+ sensitization was significantly inhibited. 6. These findings are consistent with a role for Rho or Rho-like proteins in agonist-induced increase in Ca2+ sensitivity of force production in intact and permeabilized intestinal smooth muscle.
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