ArticleThe Journal of physiology1996
Ca(2+)- and GTP-dependent exocytosis in mouse pancreatic beta-cells involves both common and distinct steps.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 69 citations in OpenAlex.
- Hypertonicity during a rapid rise in D-glucose mediates first-phase insulin secretion.Frontiers in endocrinology · 2024Article
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Cooling reduces cAMP-stimulated exocytosis and adiponectin secretion at a Ca2+-dependent step in 3T3-L1 adipocytes.PloS one · 2015Article
- PKA-independent cAMP stimulation of white adipocyte exocytosis and adipokine secretion: modulations by Ca2+ and ATP.The Journal of physiology · 2014Article
- Exocyst sec5 regulates exocytosis of newcomer insulin granules underlying biphasic insulin secretion.PloS one · 2013Article
- Unifying concepts in stimulus-secretion coupling in endocrine cells and some implications for therapeutics.Advances in physiology education · 2009Review
- Protein kinase activation increases insulin secretion by sensitizing the secretory machinery to Ca2+.The Journal of general physiology · 2004Article
- Insulin secretion: a high-affinity Ca2+ sensor after all?The Journal of general physiology · 2004Article
- Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica.The Journal of physiology · 2001Article
- Differential regulation of granule-to-granule and granule-to-plasma membrane fusion during secretion from rat pituitary lactotrophs.The Journal of cell biology · 2000Article
- Quantal analysis of 5-hydroxytryptamine release from mouse pancreatic beta-cells.The Journal of physiology · 1999Article
- Cellular activation by Ca2+ release from stores in the endoplasmic reticulum but not by increased free Ca2+ in the cytosol.The Biochemical journal · 1999Article
- The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Post-priming actions of ATP on Ca2+-dependent exocytosis in pancreatic beta cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Ca2+-induced exocytosis in individual human neutrophils: high- and low-affinity granule populations and submaximal responses.The EMBO journal · 1998Article
- Ca2+-independent insulin exocytosis induced by alpha-latrotoxin requires latrophilin, a G protein-coupled receptor.The EMBO journal · 1998Article
- The first C2 domain of synaptotagmin is required for exocytosis of insulin from pancreatic beta-cells: action of synaptotagmin at low micromolar calcium.The EMBO journal · 1997Article
- Rapid ATP-dependent priming of secretory granules precedes Ca(2+)-induced exocytosis in mouse pancreatic B-cells.The Journal of physiology · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
1. The effects of GTP and Ca2+ on secretion from single pancreatic beta-cells were studied using capacitance measurements as an indicator of exocytosis. 2. GTP or GTP gamma S produced a concentration-dependent increase in cell capacitance in the absence of intracellular calcium. There was no effect of cyclic AMP or BAPTA an GTP-induced secretion. 3. In the absence of GTP, the relationship between intracellular calcium concentration and the maximum rate of secretion was fitted by the Hill equation with a slope factor of 2.5 and half-maximal activation at 1.6 microM intracellular Ca2+. Similar values were obtained in the presence of GTP gamma S, suggesting GTP does not alter the sensitivity of the secretory machinery to Ca2+. 4. GDP beta S alone had no effect on cell capacitance but caused a dose-dependent inhibition of exocytosis induced by infusion of either GTP gamma S or Ca2+, suggesting both stimuli involve G-protein activation. GDP beta S was without effect on exocytosis evoked by depolarization-mediated Ca2+ entry. 5. The time course of exocytosis following rapid elevation of GTP gamma S by photolysis of a caged precursor was dependent on the intracellular Ca2+ and cyclic AMP concentrations. 6. Our results are interpreted in terms of a model in which the secretory pathways stimulated by Ca2+ and GTP contain both common and separate parts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.