ArticleThe Journal of physiology1996
Properties of the cAMP-activated C1- current in choroid plexus epithelial cells isolated from the rat.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Mechanisms of cerebrospinal fluid secretion by the choroid plexus epithelium: Application to various intracranial pathologies.Clinical anatomy (New York, N.Y.) · 2025Review
- Cerebrospinal fluid pH regulation.Pflugers Archiv : European journal of physiology · 2024Review
- Organization of the ventricular zone of the cerebellum.Frontiers in cellular neuroscience · 2022Review
- Inward-rectifier chloride currents in Reissner's membrane epithelial cells.Biochemical and biophysical research communications · 2010Article
- Ion channel diversity, channel expression and function in the choroid plexuses.Cerebrospinal fluid research · 2007Article
- Water and solute secretion by the choroid plexus.Pflugers Archiv : European journal of physiology · 2007Review
- Molecular mechanisms of cerebrospinal fluid production.Neuroscience · 2004Review
- Inward-rectifying anion channels are expressed in the epithelial cells of choroid plexus isolated from ClC-2 'knock-out' mice.The Journal of physiology · 2002Article
- Chloride transport in microperfused interlobular ducts isolated from guinea-pig pancreas.The Journal of physiology · 2002Article
- The choroid plexuses and the barriers between the blood and the cerebrospinal fluid.Cellular and molecular neurobiology · 2000Review
- The chloride channel ClC-2 contributes to the inwardly rectifying Cl- conductance in cultured porcine choroid plexus epithelial cells.The Journal of physiology · 2000Article
- Activation of cAMP-dependent C1- currents in guinea-pig paneth cells without relevant evidence for CFTR expression.The Journal of physiology · 1998Article
- Inhibition of the inward-rectifying Cl- channel in rat choroid plexus by a decrease in extracellular pH.The Journal of physiology · 1997Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
1. This study used whole-cell patch clamp and RNA in situ hybridization experiments to determine whether the cAMP-activated C1- current expressed in choroid plexus epithelial cells was carried by the cystic fibrosis transmembrane conductance regulator (CFTR) channel. 2. In patch clamp experiments, inclusion of 0.25 mM cAMP and 375 protein kinase A catalytic subunit (PKA) in the electrode solution caused activation of an inwardly rectifying current (21/23 cells). This current was C1- selective, since the current reversal potential (Erev) was -31 +/- 3 mV with equilibrium potential values for C1- (EC1) and Na+ (ENa) of -44 and 0 mV, respectively. 3. In anion substitution experiments, the relative anion permeability sequence for the inward rectifier was: I- (3.5) > HCO3-(1.5) = C1-(1.0) > Br-(0.6) > aspartate (0.2). 4. The inward rectifier was sensitive to inhibition by a range of known channel inhibitors, including: glibenclamide (100 microns), DIDS (100 and 500 microns), NPPB (100 microns) and Ba2+ (1 mM). 5. In RNA in situ hybridization experiments, using two independent rat CFTR cRNA probes, expression of CFTR could not be detected in epithelial cells from the rat choroid plexus. 6. In conclusion, the cAMP-dependent whole-cell C1- current present in choroid plexus epithelial cells from the rat has properties which are distinctly different from those of CFTR.
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