ArticleThe Journal of cell biology1996
Delivery of newly synthesized tubulin to rapidly growing distal axons of sympathetic neurons in compartmented cultures.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Imaging specific newly synthesized proteins within cells by fluorescence resonance energy transfer.Chemical science · 2017Article
- Compartmentalized Platforms for Neuro-Pharmacological Research.Current neuropharmacology · 2016Review
- Molecular Biology and Clinical Mitigation of Cancer Treatment-Induced Neuropathy.Clinical Medicine Insights. Oncology · 2016Review
- Augmentation of Cav1 channel current and action potential duration after uptake of S100A1 in sympathetic ganglion neurons.American journal of physiology. Cell physiology · 2009Article
- Production of compartmented cultures of rat sympathetic neurons.Nature protocols · 2009Article
- Progressive motor neuronopathy: a critical role of the tubulin chaperone TBCE in axonal tubulin routing from the Golgi apparatus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Direct evidence for coherent low velocity axonal transport of mitochondria.The Journal of cell biology · 2006Article
- Compartment volume influences microtubule dynamic instability: a model study.Biophysical journal · 2006Article
- A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
- Slow axonal transport of neurofilament protein in cultured neurons.The Journal of cell biology · 1999Article
- Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Cytoplasmic dynein and microtubule transport in the axon: the action connection.Molecular neurobiologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Growing axons receive a substantial supply of tubulin and other proteins delivered from sites of synthesis in the cell body by slow axonal transport. To investigate the mechanism of tubulin transport most previous studies have used in vitro models in which the transport of microtubules can be visualized during brief periods of growth. To investigate total tubulin transport in neurons displaying substantial growth over longer periods, we used rat sympathetic neurons in compartmented cultures. Tubulin synthesized during pulses of [35S]methionine was separated from other proteins by immunoprecipitation with monoclonal antibodies to alpha and beta tubulin, further separated on SDS-PAGE, and quantified by phosphorimaging. Results showed that 90% of newly synthesized tubulin moved into the distal axons within 2 d. Furthermore, the leading edge of tubulin was transported at a velocity faster than 4 mm/d, more than four times the rate of axon elongation. This velocity did not diminish with distance from the cell body, suggesting that the transport system is capable of distributing newly synthesized tubulin to growth cones throughout the axonal tree. Neither diffusion nor the an mass transport of axonal microtubules can account for the velocity and magnitude of tubulin transport that was observed. Thus, it is likely that most of the newly synthesized tubulin was supplied to the growing axonal tree in subunit form such as a heterodimer or an oligomer considerably smaller than a microtubule.
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Registered trials
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