ArticleThe Journal of cell biology1996
RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 175 citations in OpenAlex.
- Ran modulates allosteric crosstalk between importin β surfaces.Nature communications · 2025Article
- Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells.Cell structure and function · 2024Article
- RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases.Cancers · 2023Review
- Review
- Recognition by host nuclear transport proteins drives disorder-to-order transition in Hendra virus V.Scientific reports · 2018Article
- Sumoylation of the GTPase Ran by the RanBP2 SUMO E3 Ligase Complex.The Journal of biological chemistry · 2015Article
- Review
- Increased cathepsin D protein expression is a biomarker for osteosarcomas, pulmonary metastases and other bone malignancies.Oncotarget · 2015Article
- Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin.The Journal of biological chemistry · 2011Article
- Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells.Chromosoma · 2010Article
- Article
- Identification of protein cofactors necessary for sequence-specific plasmid DNA nuclear import.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import.The Journal of biological chemistry · 2009Article
- The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear import.Molecular biology of the cell · 2008Article
- Regulation of nuclear lamin polymerization by importin alpha.The Journal of biological chemistry · 2008Article
- The unfolded protein response transducer Ire1p contains a nuclear localization sequence recognized by multiple beta importins.Molecular biology of the cell · 2006Article
- RanBP2 modulates Cox11 and hexokinase I activities and haploinsufficiency of RanBP2 causes deficits in glucose metabolism.PLoS genetics · 2006Article
- Identification and characterization of the Arabidopsis orthologs of nuclear transport factor 2, the nuclear import factor of ran.Plant physiology · 2006Article
- The positively charged termini of L2 minor capsid protein required for bovine papillomavirus infection function separately in nuclear import and DNA binding.Journal of virology · 2004Article
- The l2 minor capsid protein of human papillomavirus type 16 interacts with a network of nuclear import receptors.Journal of virology · 2004Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Three factors have been identified that reconstitute nuclear protein import in a permeabilized cell assay: the NLS receptor, p97, and Ran/TC4. Ran/TC4, in turn, interacts with a number of proteins that are involved in the regulation of GTP hydrolysis or are components of the nuclear pore. Two Ran-binding proteins, RanBP1 and RanBP2, form discrete complexes with p97 as demonstrated by immunoadsorption from HeLa cell extracts fractionated by gel filtration chromatography. A > 400-kD complex contains p97, Ran, and RanBP2. Another complex of 150-300 kD was comprised of p97, Ran, and RanBP1. This second trimeric complex could be reconstituted from recombinant proteins. In solution binding assays, Ran-GTP bound p97 with high affinity, but the binding of Ran-GDP to p97 was undetectable. The addition of RanBP1 with Ran-GDP or Ran-GTP increased the affinity of both forms of Ran for p97 to the same level. Binding of Ran-GTP to p97 dissociated p97 from immobilized NLS receptor while the Ran-GDP/RanBP1/p97 complex did not dissociate from the receptor. In a digitonin-permeabilized cell docking assay, RanBP1 stabilizes the receptor complex against temperature-dependent release from the pore. When added to an import assay with recombinant NLS receptor, p97 and Ran-GDP, RanBP1 significantly stimulates transport. These results suggest that RanBP1 promotes both the docking and translocation steps in nuclear protein import by stabilizing the interaction of Ran-GDP with p97.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.