ArticleThe Journal of cell biology1996
Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
86 citing papers in PubMed.
- The TUBG meshwork is associated with centromere dynamics and micronuclear organization.Communications biology · 2026Article
- Phosphoinositide signaling at the cytoskeleton in the regulation of cell dynamics.Cell death & disease · 2025Review
- Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.Research square · 2024Article
- Microtubule-Targeting Agents: Disruption of the Cellular Cytoskeleton as a Backbone of Ovarian Cancer Therapy.Advances in experimental medicine and biology · 2024Review
- Acute irradiation induces a senescence-like chromatin structure in mammalian oocytes.Communications biology · 2023Article
- Loss of sister kinetochore co-orientation and peri-centromeric cohesin protection after meiosis I depends on cleavage of centromeric REC8.Developmental cell · 2021Article
- The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle.Open biology · 2020Review
- Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.Nucleus (Austin, Tex.) · 2019Article
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- Spo13 prevents premature cohesin cleavage during meiosis.Wellcome open research · 2019Article
- Applications of the CRISPR/Cas system beyond gene editing.Biology methods & protocols · 2018Review
- Article
- CRISPR/Cas9 in Genome Editing and Beyond.Annual review of biochemistry · 2016Review
- Organization of nuclear architecture during adipocyte differentiation.Nucleus (Austin, Tex.) · 2016Review
- Chromosomes at Work: Organization of Chromosome Territories in the Interphase Nucleus.Journal of cellular biochemistry · 2016Article
- Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines.Molecular biology of the cell · 2015Article
- Cell type specific alterations in interchromosomal networks across the cell cycle.PLoS computational biology · 2014Article
- Targeting microtubules by natural agents for cancer therapy.Molecular cancer therapeutics · 2014Review
- Kif18A and chromokinesins confine centromere movements via microtubule growth suppression and spatial control of kinetochore tension.Developmental cell · 2012Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have constructed a fluorescent alpha-satellite DNA-binding protein to explore the motile and mechanical properties of human centromeres. A fusion protein consisting of human CENP-B coupled to the green fluorescent protein (GFP) of A. victoria specifically targets to centromeres when expressed in human cells. Morphometric analysis revealed that the alpha-satellite DNA domain bound by CENPB-GFP becomes elongated in mitosis in a microtubule-dependent fashion. Time lapse confocal microscopy in live mitotic cells revealed apparent elastic deformations of the central domain of the centromere that occurred during metaphase chromosome oscillations. These observations demonstrate that the interior region of the centromere behaves as an elastic element that could play a role in the mechanoregulatory mechanisms recently identified at centromeres. Fluorescent labeling of centromeres revealed that they disperse throughout the nucleus in a nearly isometric expansion during chromosome decondensation in telophase and early G1. During interphase, centromeres were primarily stationary, although motility of individual or small groups of centromeres was occasionally observed at very slow rates of 7-10 microns/h.
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