ArticleThe American journal of pathology1996
Growth and differentiation in culture of clonogenic hepatocytes that express both phenotypes of hepatocytes and biliary epithelial cells.
Article in The American journal of pathology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 77 citations in OpenAlex.
- "Small Hepatocytes" in the Liver.Cells · 2023Review
- Art of Making Artificial Liver: Depicting Human Liver Biology and Diseases in Mice.Seminars in liver disease · 2020Review
- Hepato-biliary profile of potential candidate liver progenitor cells from healthy rat liver.World journal of gastroenterology · 2012Article
- Management of Liver Failure: From Transplantation to Cell-Based Therapy.Cell medicine · 2011Article
- Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver.Cell stress & chaperones · 2010Article
- Endogenous and transplanted small hepatocytes in retrorsine-treated/partially hepatectomized rat liver show differences in growth, phenotype, and proximity to clusters of gamma-glutamyl transpeptidase-positive host hepatocytes.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Identification of vitamin A-free cells in a stellate cell-enriched fraction of normal rat liver as myofibroblasts.Histochemistry and cell biology · 2007Article
- Multipotent adult progenitor cell and stem cell plasticity.Stem cell reviews · 2005Review
- Near completely humanized liver in mice shows human-type metabolic responses to drugs.The American journal of pathology · 2004Article
- Pleiotrophin/heparin-binding growth-associated molecule as a mitogen of rat hepatocytes and its role in regeneration and development of liver.The American journal of pathology · 2002Article
- Size-dependent in vivo growth potential of adult rat hepatocytes.The American journal of pathology · 2001Article
- The in vitro differentiating capacity of nonparenchymal epithelial cells derived from adult porcine livers.The American journal of pathology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cell fraction containing small hepatocytes and nonparenchymal cells was isolated from the adult rat liver and was cultured in the presence of vitamin C. epidermal growth factor, nicotinamide, and dimethylsulfoxide. All of the small hepatocytes that had attached to a dish expressed hepatocytic phenotypes such as albumin, transferrin, and cytokeratin (CK)8 and CK18 but not biliary markers such as BD1, CK7, and CK19. Single hepatocytes started to proliferate and grew into colonies. The growth potential was variable among the cells, the highest case being that a single cell produced a colony containing over 100 cells in 10 days. The hepatocytes in the colony developed through a proliferation phase and then a differentiation phase. They showed very high bromodeoxyuridine labeling indexes during the first 7 days (proliferation phase), which gradually decreased thereafter. Phenotypic alterations took place at 7 to 10 days, and some hepatocytes started to express mature hepatocyte markers and biliary markers (differentiation phase). The presence of cells that coexpress albumin and biliary markers (CK7 and CK19) was demonstrated by double immunocytochemistry. In addition, cells were identified that ceased to express albumin and in turn were positive for CK19 or CK7. Therefore, the colony was considered to contain liver progenitor-like cells that can differentiate during culture into cells expressing phenotypes of mature hepatocytes or biliary epithelial cells.
Indexed as
Identifiers
8909249PMC1865270W124367642What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.