ArticleJournal of clinical microbiology1996
Rapid identification of Candida species by DNA fingerprinting with PCR.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 99 citations in OpenAlex.
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- Budding off: bringing functional genomics to Candida albicans.Briefings in functional genomics · 2016Review
- Antifungal Susceptibility in Serum and Virulence Determinants of Candida Bloodstream Isolates from Hong Kong.Frontiers in microbiology · 2016Article
- Rapid identification of Sporothrix species by T3B fingerprinting.Journal of clinical microbiology · 2012Article
- Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation.BMC microbiology · 2009Article
- CandidaDB: a multi-genome database for Candida species and related Saccharomycotina.Nucleic acids research · 2008Article
- Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.Journal of clinical microbiology · 2006Article
- New microsatellite multiplex PCR for Candida albicans strain typing reveals microevolutionary changes.Journal of clinical microbiology · 2005Article
- Study of molecular epidemiology of candidiasis in portugal by PCR fingerprinting of Candida clinical isolates.Journal of clinical microbiology · 2004Article
- Highly polymorphic microsatellite for identification of Candida albicans strains.Journal of clinical microbiology · 2003Article
- Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.Journal of clinical microbiology · 2003Article
- Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.Journal of clinical microbiology · 2001Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Application of nucleic acid amplification in clinical microbiology.Molecular biotechnology · 1999Review
- High-frequency, in vitro reversible switching of Candida lusitaniae clinical isolates from amphotericin B susceptibility to resistance.Antimicrobial agents and chemotherapy · 1999Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
DNA polymorphisms in different species and strains of the genus Candida were assessed by amplifying genomic DNA with single nonspecific primers. This PCR method employed an arbitrary primer (the 10-mer AP3), a primer derived from the intergenic spacer regions (T3B), and the microsatellite primers (GTG)5 and (AC)10. Distinctive and reproducible sets of amplification products were observed for 26 different Candida and 8 other fungal species. The numbers and sizes of the amplification products were characteristic for each species. All yeast species tested could be clearly distinguished by their amplification patterns. With all primers, PCR fingerprints also displayed intraspecies variability. However, PCR profiles obtained from different strains of the same species were far more similar than those derived from different Candida species. By comparing species-specific PCR fingerprints of clinical isolates with those of reference strains, clinical isolates could be identified to the species level even if they could not be identified by routine biochemical methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.