ArticleJournal of clinical microbiology1996
Novel, ligation-dependent PCR assay for detection of hepatitis C in serum.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Detection of the phosphorothioate oligonucleotide fomivirsen using a ligase detection reaction with polymerase chain reaction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia.Diagnostics (Basel, Switzerland) · 2023Review
- Magnetic Bead Processing Enables Sensitive Ligation-Based Detection of HIV Drug Resistance Mutations.Analytical chemistry · 2022Article
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications.Mutation research. Reviews in mutation research · 2017Review
- Article
- Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase.Nucleic acids research · 2014Article
- Nucleic acid amplification: Alternative methods of polymerase chain reaction.Journal of pharmacy & bioallied sciences · 2013Review
- Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.Nucleic acids research · 2013Article
- Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification.Nucleic acids research · 2012Article
- Analysis of microRNA signatures using size-coded ligation-mediated PCR.Nucleic acids research · 2011Article
- Development of a single-step subtraction method for eukaryotic 18S and 28S ribonucleic acids.Journal of biomedicine & biotechnology · 2011Article
- A simple way to evaluate self-designed probes for tumor specific Multiplex Ligation-dependent Probe Amplification (MLPA).BMC research notes · 2010Article
- Emerging molecular assays for detection and characterization of respiratory viruses.Clinics in laboratory medicine · 2009Review
- A method for high-throughput gene expression signature analysis.Genome biology · 2006Article
- Use of ramification amplification assay for detection of Escherichia coli O157:H7 and other E. coli Shiga toxin-producing strains.Journal of clinical microbiology · 2005Article
- A versatile assay for high-throughput gene expression profiling on universal array matrices.Genome research · 2004Article
- Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification.Nucleic acids research · 2002Article
- Detection of Chlamydia trachomatis by isothermal ramification amplification method: a feasibility study.Journal of clinical microbiology · 2002Article
- RNA-templated DNA ligation for transcript analysis.Nucleic acids research · 2001Article
- Cooperative oligonucleotides mediating direct capture of hepatitis C virus RNA from serum.Journal of clinical microbiology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A simple, sensitive, and specific ligation-dependent PCR (LD-PCR) method for the detection of hepatitis C virus (HCV) RNA in serum is described. The assay uses two DNA capture probes for RNA isolation and two DNA hemiprobes for subsequent PCR. Each capture probe has a 3' sequence complementary to the conserved 5' untranslated region of HCV RNA and a biotin moiety at the 5' end capable of interacting with streptavidin-coated paramagnetic beads. Each hemiprobe contains a sequence complementary to the 5' untranslated region in juxtaposition to one another and a common sequence for PCR primer binding. In guanidinium thiocyanate solutions, the capture probes and the hemiprobes form a hybrid with their target, and the hybrid can be isolated from serum by the binding of the capture probes to the paramagnetic beads in the presence of a magnetic field. The hemiprobes can then be linked to each other by incubation with T4 DNA ligase to form a full probe that serves as a template for a PCR. When serial 10-fold dilutions of synthetic HCV RNA (10(7) to 10 molecules) were tested, there was a good correlation between the amount of PCR product and the initial number of RNA molecules, with a sensitivity of 100 HCV RNA molecules per reaction. Twenty-four specimens that had been tested by either a branched DNA probe (bDNA) assay (13 specimens) or a reverse transcription PCR (RT-PCR) assay (11 specimens) were also analyzed by LD-PCR. The results showed a good correlation among LD-PCR, RT-PCR, and the bDNA assay. However, both LD-PCR and RT-PCR were more sensitive than the bDNA assay when the HCV titer was low.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.