ArticleJournal of cancer research and clinical oncology1996
Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients.
Article in Journal of cancer research and clinical oncology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 89 citations in OpenAlex.
- Detection of circulating tumour cells in patients with breast or ovarian cancer by molecular cytogenetics.British journal of cancer · 1999Trial
- Detection of Minimal Residual Disease in the Peripheral Blood of Breast Cancer Patients, with a Multi Marker (MGB-1, MGB-2, CK-19 and NY-BR-1) Assay.Breast cancer (Dove Medical Press) · 2021Article
- Phosphorylated EGFR and PI3K/Akt signaling kinases are expressed in circulating tumor cells of breast cancer patients.Breast cancer research : BCR · 2008Article
- Measurement of thyroglobulin mRNA in peripheral blood as an adjunctive test for monitoring thyroid cancer.Molecular pathology : MP · 2003Article
- Lack of correlation between expression of human mammaglobin mRNA in peripheral blood and known prognostic factors for breast cancer patients.Cancer science · 2003Article
- Article
- T cell memory, anergy and immunotherapy in breast cancer.Journal of mammary gland biology and neoplasia · 2002Review
- Detection of micrometastatic tumor cells in pN0 lymph nodes of patients with completely resected nonsmall cell lung cancer: impact on recurrence and Survival.Annals of surgery · 2002Article
- Molecular biological markers and micrometastasis in resected non-small-cell lung cancer. Prognostic implications.The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi · 2001Article
- Detection of breast cancer cell contamination in leukapheresis product by real-time quantitative polymerase chain reaction.Bone marrow transplantation · 2001Article
- Interference of cytokeratin-20 and mammaglobin-reverse-transcriptase polymerase chain assays designed for the detection of disseminated cancer cells.Medical oncology (Northwood, London, England) · 2001Article
- Expression of human milk fat globulin proteins in cells of haemopoietic origin.British journal of cancer · 2000Article
- Molecular diagnostic tests in the diagnosis and management of thyroid carcinoma.Reviews in endocrine & metabolic disorders · 2000Review
- Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes.British journal of cancer · 1999Article
- Tumour cell detection in G-CSF mobilised stem cell harvests of patients with breast cancer.Medical oncology (Northwood, London, England) · 1999Article
- Specificity of reverse transcriptase polymerase chain reaction assays designed for the detection of circulating cancer cells is influenced by cytokines in vivo and in vitro.British journal of cancer · 1998Article
- Reverse transcriptase/polymerase chain reaction analysis of parathyroid hormone-related protein for the detection of tumor cell dissemination in the peripheral blood and bone marrow of patients with breast cancer.Journal of cancer research and clinical oncology · 1997Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A two-step reverse-transcriptase-based polymerase chain reaction (PCR) with nested primer pairs was developed to amplify sensitive and specific cytokeratin-19 (CK-19) mRNA sequences from human breast cancer cells. No CK-19 pseudogene interference was seen. The larger DNA-derived amplification products could be clearly discriminated from mRNA-derived products. The CK-19 message was not amplified from bone marrow or blood of healthy volunteers and patients with haematological malignancies nor from myeloid and lymphoid cell lines. Breast cancer cells were diluted in buffy coat cells up to 10(-6) and CK-19 mRNA sought by PCR. The CK-19 message was detected in 14 of 26 blood samples and 14 of 24 marrow samples but in neither of two peripheral blood stem cell samples taken from 35 breast cancer patients. By sequence-analysis control of two of these samples and two cell lines, the amplified DNA fragments were confirmed to be homologous with the CK-19 sequence. The CK-19 message was further sought in matched blood/marrow samples taken from 13 untreated women in the same cohort at the time of diagnosis. In 3 of these, CK-19 RNA was detected in blood and marrow and, in 3 others, only in blood, but never in marrow alone. The results show that CK-19 assay by reverse transcriptase/PCR is a sensitive and specific technique for the detection of cancer cells in bone marrow and blood. It could be helpful in diagnosis and monitoring of metastatic breast cancer and detection of micrometastases. This should be evaluated on larger numbers of patients, with different clinical samples and epithelial malignancies.
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