ArticleMolecular biology of the cell1996
The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 189 citations in OpenAlex.
- Cephalomannine as in vitro and in vivo anticancer agent in mesothelioma cells: mechanisms of action.BMC cancer · 2025Article
- KIF2C is essential for meiosis and manchette dynamics in male mice.Frontiers in cell and developmental biology · 2025Article
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle.eLife · 2023Article
- MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles.Molecular biology of the cell · 2023Article
- Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules.Nature communications · 2022Article
- Article
- Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules.Current biology : CB · 2022Article
- Dynamics of kinetochore structure and its regulations during mitotic progression.Cellular and molecular life sciences : CMLS · 2020Review
- Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.eLife · 2020Article
- Blocking Mitotic Exit of Ovarian Cancer Cells by Pharmaceutical Inhibition of the Anaphase-Promoting Complex Reduces Chromosomal Instability.Neoplasia (New York, N.Y.) · 2019Article
- Anaphase Chromosomes in Crane-Fly Spermatocytes Treated With Taxol (Paclitaxel) Accelerate When Their Kinetochore Microtubules Are Cut: Evidence for Spindle Matrix Involvement With Spindle Forces.Frontiers in cell and developmental biology · 2018Article
- Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.Current biology : CB · 2017Article
- Article
- Review
- Aurora-A regulates MCRS1 function during mitosis.Cell cycle (Georgetown, Tex.) · 2016Article
- Using Photobleaching to Measure Spindle Microtubule Dynamics in Primary Cultures of Dividing Drosophila Meiotic Spermatocytes.Journal of biomolecular techniques : JBT · 2015Article
- How Taxol/paclitaxel kills cancer cells.Molecular biology of the cell · 2014Article
- Prime movers: the mechanochemistry of mitotic kinesins.Nature reviews. Molecular cell biology · 2014Review
- A mathematical model of force generation by flexible kinetochore-microtubule attachments.Biophysical journal · 2014Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
During metaphase and anaphase in newt lung cells, tubulin subunits within the kinetochore microtubule (kMT) lattice flux slowly poleward as kMTs depolymerize at their minus-ends within in the pole. Very little is known about how and where the force that moves the tubulin subunits poleward is generated and what function it serves during mitosis. We found that treatment with the drug taxol (10 microM) caused separated centrosomes in metaphase newt lung cells to move toward one another with an average velocity of 0.89 microns/min, until the interpolar distance was reduced by 22-62%. This taxol-induced spindle shortening occurred as kMTs between the chromosomes and the poles shortened. Photoactivation of fluorescent marks on kMTs revealed that taxol inhibited kinetochore microtubule assembly/disassembly at kinetochores, whereas minus-end MT disassembly continued at a rate typical of poleward flux in untreated metaphase cells. This poleward flux was strong enough to stretch the centromeric chromatin between sister kinetochores as much as it is stretched in control metaphase cells. In anaphase, taxol blocked kMT disassembly/assembly at the kinetochore whereas minus-end disassembly continued at a rate similar to flux in control cells (approximately 0.2 microns/min). These results reveal that the mechanism for kMT poleward flux 1) is not dependent on kMT plus-end dynamics and 2) produces pulling forces capable of generating tension across the centromeres of bioriented chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.