ArticleProtein science : a publication of the Protein Society1996
Cation selective promotion of tubulin polymerization by alkali metal chlorides.
Article in Protein science : a publication of the Protein Society, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 61 citations in OpenAlex.
- Modulation of microtubule dynamics by monovalent ions.PNAS nexus · 2025Article
- Taurine Is Covalently Incorporated into Alpha-Tubulin.Journal of proteome research · 2020Article
- Microtubule Destabilization Paves the Way to Parkinson's Disease.Molecular neurobiology · 2017Review
- HeLife · 2017Article
- An Emerging Role for Tubulin Isotypes in Modulating Cancer Biology and Chemotherapy Resistance.International journal of molecular sciences · 2017Review
- Tubulin Dimer Reversible Dissociation: AFFINITY, KINETICS, AND DEMONSTRATION OF A STABLE MONOMER.The Journal of biological chemistry · 2016Article
- STAQ: A route toward low power, multicolor nanoscopy.Microscopy research and technique · 2015Article
- The Ca2+-activated Cl- channel ANO1/TMEM16A regulates primary ciliogenesis.Molecular biology of the cell · 2014Article
- Article
- Production of reliable MALDI spectra with quality threshold clustering of replicates.Journal of the American Society for Mass Spectrometry · 2011Article
- Effective charge measurements reveal selective and preferential accumulation of anions, but not cations, at the protein surface in dilute salt solutions.Protein science : a publication of the Protein Society · 2011Article
- Phosphorylation of voltage-dependent anion channel by serine/threonine kinases governs its interaction with tubulin.PloS one · 2011Article
- Direct measurement of energy fluxes from mitochondria into cytoplasm in permeabilized cardiac cells in situ: some evidence for Mitochondrial Interactosome.Journal of bioenergetics and biomembranes · 2009Article
- Tubulin and its prokaryotic homologue FtsZ: a structural and functional comparison.Science progress · 2009Review
- Ion-specific modulation of protein interactions: anion-induced, reversible oligomerization of a fusion protein.Protein science : a publication of the Protein Society · 2009Article
- Tubulin binding blocks mitochondrial voltage-dependent anion channel and regulates respiration.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Regulation of respiration in brain mitochondria and synaptosomes: restrictions of ADP diffusion in situ, roles of tubulin, and mitochondrial creatine kinase.Molecular and cellular biochemistry · 2008Article
- Nitrosoureas inhibit the stathmin-mediated migration and invasion of malignant glioma cells.Cancer research · 2008Article
- Conformational analysis of the carboxy-terminal tails of human beta-tubulin isotypes.Biophysical journal · 2008Article
- Evaluating reproducibility and similarity of mass and intensity data in complex spectra--applications to tubulin.Journal of the American Society for Mass Spectrometry · 2008Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A role for charge-based interactions in protein stability at the monomer or dimer level is well known. We show here that such interactions can also be important for the higher-order structures of microtubule assembly. Alkali metal chlorides increase the rate of polymerization of pure tubulin driven by either taxol or dimethyl sulfoxide. The effect is cation selective, exhibiting a sequence Na+ > K+ > Li+ > Cs+, with optimal concentrations for Na+ at approximately 160 mM. Hofmeister anion effects are additive with these rate stimulations. Sodium is less potent than guanidinium ion stimulation reported previously, but produces a larger fraction of normal microtubules. Alkali metal cations lower the critical concentration by a factor of approximately 2, produce cold reversible polymers whose formation is sensitive to podophyllotoxin inhibition, increase the fraction of polymers present as microtubules from approximately 0.9 to 0.99, and reverse or prevent urea-induced depolymerization of microtubules. In the presence of microtubule-associated proteins, the promotion of polymerization is no longer cation selective. In the polymerization of tubulin S, in which the acidic C termini of both monomers have been cleaved, the cation enhancement is markedly decreased, although selective persists. Because the selectivity sequence is similar to that of the coil/helix transition of polyglutamic acid, we suggest that a major part, although not all, of the cation selective enhancement of polymerization results from shielding of the glutamate-rich C termini of the tubulin monomers.
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