ArticleJournal of clinical microbiology1996
Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Synergistic potentiation of antibiotics by chamomile phytochemicals against multidrug-resistant Helicobacter pylori.Gut pathogens · 2025Article
- Polymorphism of virulence genes and biofilm associated with in vitro induced resistance to clarithromycin in Helicobacter pylori.Gut pathogens · 2023Article
- The -160 (C>A) CDH1 Gene Promoter Polymorphism and Its Relationship with Survival of Patients with Gastric Cancer in KurdistanAsian Pacific journal of cancer prevention : APJCP · 2017Article
- An In Silico Approach for Identification of the Pathogenic Species, Helicobacter pylori and Its Relatives.Indian journal of microbiology · 2016Article
- Milk of livestock as a possible transmission route of Helicobacter pylori infection.Gastroenterology and hepatology from bed to bench · 2015Article
- Diagnosis of Helicobacter pylori: what should be the gold standard?World journal of gastroenterology · 2014Review
- Presence of Helicobacter pylori in subgingival plaque of periodontitis patients with and without dyspepsia, detected by polymerase chain reaction and culture.Journal of Indian Society of Periodontology · 2012Article
- Specific and sensitive detection of H. pylori in biological specimens by real-time RT-PCR and in situ hybridization.PloS one · 2008Article
- Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60.Helicobacter · 2008Article
- Helicobacter pylori detection and antimicrobial susceptibility testing.Clinical microbiology reviews · 2007Review
- Detection of Helicobacter pylori in gastric mucosa of patients with gastroduodenal diseases by PCR-restriction analysis using the RNA polymerase gene (rpoB).Journal of clinical microbiology · 2003Article
- PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples.Journal of clinical microbiology · 2001Article
- Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues.Journal of clinical microbiology · 1999Article
- Helicobacter pylori in dental plaque: a comparison of different PCR primer sets.Digestive diseases and sciences · 1999Article
- Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori.Journal of clinical microbiology · 1998Article
- Helicobacter pylori.Clinical microbiology reviews · 1997Review
- Rapid and effective method for preparation of fecal specimens for PCR assays.Journal of clinical microbiology · 1997Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PCR primer set Hp1-Hp2, which amplifies a 109-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been widely used for the detection of H. pylori in clinical specimens. We have examined 34 stool samples and 50 human tissue samples from H. pylori-infected and uninfected patients, five human leukocyte samples, and one human cell line by this PCR method. All of these specimens produced a 109-bp PCR product. When Escherichia coli DNA was used as the template, several nonspecific bands, but not the 109-bp band, were observed. No PCR products were generated when DNA samples from five different fungi were used as templates. These results indicate that this 109-bp PCR product was amplified from the human genome. The 109-bp PCR product generated from various clinical specimens also hybridized with the probe pHp, corresponding to a region internal to the PCR product of Hp1-Hp2. We conclude that the 16S rRNA gene PCR with the primer set Hp1-Hp2 is not specific and cannot be used to detect H. pylori in clinical specimens.
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