ArticleJournal of clinical microbiology1996
Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Microbiological Analysis Following Periodontal Treatment in Individuals with Bronchiectasis.Microorganisms · 2026Article
- A capsular polysaccharide from a healthy human microbiota member activates a Lag-3-NK cell axis to restrain colon cancer and augment immunotherapy.Cell reports · 2025Article
- Antibacterial Effect of Silver Nanoparticles against Oral Biofilms in Subjects with Motor and Intellectual Disabilities.Journal of functional biomaterials · 2024Article
- Bactericidal Activity of Silver Nanoparticles on Oral Biofilms Related to Patients with and without Periodontal Disease.Journal of functional biomaterials · 2023Article
- Oral hygiene, mouthwash usage and cardiovascular mortality during 18.8 years of follow-up.British dental journal · 2023Article
- Article
- Article
- Localization and density of Porphyromonas gingivalis and Tannerella forsythia in gingival and subgingival granulation tissues affected by chronic or aggressive periodontitis.Scientific reports · 2018Article
- Relationship between the burden of major periodontal bacteria and serum lipid profile in a cross-sectional Japanese study.BMC oral health · 2018Article
- Higher prevalence of Epstein-Barr virus DNA in deeper periodontal pockets of chronic periodontitis in Japanese patients.PloS one · 2013Article
- Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease.BMC gastroenterology · 2012Article
- The rag locus of Porphyromonas gingivalis might arise from Bacteroides via horizontal gene transfer.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
- Bacterial adhesion of porphyromonas gingivalis on provisional fixed prosthetic materials.Dental research journal · 2010Article
- Distribution of periodontopathic bacterial species in Japanese children with developmental disabilities.BMC oral health · 2009Article
- Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.Clinical and experimental immunology · 2009Article
- Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens.Journal of clinical microbiology · 2006Article
- Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of fimA gene.Journal of clinical microbiology · 2000Article
- Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis.Journal of clinical microbiology · 1999Article
- Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients.Journal of clinical microbiology · 1999Article
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Authors and funding
2 authors.
Funding
Abstract
Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis are strongly associated with periodontitis. However, little is known about their distribution in periodontally healthy individuals, because culturing techniques are not sufficiently sensitive. A modified multiplex PCR was developed to address that question. Our method uses two species-specific forward primers in combination with a single reverse primer. These primers target variable and conserved regions of the 16S rRNA gene. Sensitivity was determined by testing serial dilutions of A. actinomycetemcomitans and P. gingivalis cells. Primer specificity was tested against (i) six A. actinomycetemcomitans strains and four P. gingivalis strains, (ii) seven different species of oral bacteria, and (iii) supra- and subgingival plaque from 20 subjects. The multiplex PCR had a lower limit of detection of 2 A. actinomycetemcomitans and 30 P. gingivalis cells. Species-specific amplicons were obtained for all A. actinomycetemcomitans and P. gingivalis strains tested and did not occur with seven other bacterial species unless A. actinomycetemcomitans and P. gingivalis were added. Neither target species was detected in supragingival plaque; A. actinomycetemcomitans was detected in one subgingival specimen, and P. gingivalis was detected in another. When plaque samples were spiked with 10 A. actinomycetemcomitans cells and 100 P. gingivalis cells, species-specific amplicons were detected. These findings show our multiplex PCR to be highly sensitive and specific while allowing simultaneous detection of A. actinomycetemcomitans and P. gingivalis. This assay has potential applications in epidemiological studies, diagnosis, treatment planning, and monitoring of periodontal pathogens.
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