ArticleThe Journal of cell biology1996
Insulin unmasks a COOH-terminal Glut4 epitope and increases glucose transport across T-tubules in skeletal muscle.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Exofacial Epitope-Specific Antibodies Detect GLUT4 Translocation in Adult Human, Rat, and Mouse Skeletal Muscle.Diabetes · 2026Article
- Centronuclear Myopathy Caused by Defective Membrane Remodelling of Dynamin 2 and BIN1 Variants.International journal of molecular sciences · 2022Review
- NLRP3 Inflammasome: Potential Role in Obesity Related Low-Grade Inflammation and Insulin Resistance in Skeletal Muscle.International journal of molecular sciences · 2021Review
- Is GLUT4 translocation the answer to exercise-stimulated muscle glucose uptake?American journal of physiology. Endocrinology and metabolism · 2021Article
- Neuregulin, an Effector on Mitochondria Metabolism That Preserves Insulin Sensitivity.Frontiers in physiology · 2020Review
- Regulation of Skeletal Muscle Glucose Transport and Glucose Metabolism by Exercise Training.Nutrients · 2019Review
- Thirty sweet years of GLUT4.The Journal of biological chemistry · 2019Review
- Mechanisms of Insulin Action and Insulin Resistance.Physiological reviews · 2018Review
- Myocyte membrane and microdomain modifications in diabetes: determinants of ischemic tolerance and cardioprotection.Cardiovascular diabetology · 2017Review
- Membrane Cholesterol in Skeletal Muscle: A Novel Player in Excitation-Contraction Coupling and Insulin Resistance.Journal of diabetes research · 2017Review
- Differential Role of Insulin/IGF-1 Receptor Signaling in Muscle Growth and Glucose Homeostasis.Cell reports · 2015Article
- Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo.Diabetes · 2013Article
- Insulin- and contraction-induced glucose transporter 4 traffic in muscle: insights from a novel imaging approach.Exercise and sport sciences reviews · 2013Review
- Immunofluorescent visualisation of focal adhesion kinase in human skeletal muscle and its associated microvasculature.Histochemistry and cell biology · 2012Article
- Kinetics of contraction-induced GLUT4 translocation in skeletal muscle fibers from living mice.Diabetes · 2010Article
- The ontogeny of insulin signaling in the preterm baboon model.Endocrinology · 2010Article
- Myotube formation on micro-patterned glass: intracellular organization and protein distribution in C2C12 skeletal muscle cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Identification of amino acid residues within the C terminus of the Glut4 glucose transporter that are essential for insulin-stimulated redistribution to the plasma membrane.The Journal of biological chemistry · 2008Article
- Structural basis of GLUT1 inhibition by cytoplasmic ATP.The Journal of general physiology · 2007Article
- Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
An improved immunogold labeling procedure was used to examine the subcellular distribution of glucose transporters in Lowricryl HM20-embedded skeletal muscle from transgenic mice overexpressing either Glut1 or Glut4. In basal muscle, Glut4 was highly enriched in membranes of the transverse tubules and the terminal cisternae of the triadic junctions. Less than 10% of total muscle Glut4 was present in the vicinity of the sarcolemmal membrane. Insulin treatment increased the number of gold particles associated with the transverse tubules and the sarcolemma by three-fold. However, insulin also increased the total Glut4 immunogold reactivity in muscle ultrathin sections by up to 1.8-fold and dramatically increased the amount of Glut4 in muscle sections as observed by laser confocal immunofluorescence microscopy. The average diameter of transverse tubules observed in longitudinal sections increased by 50% after insulin treatment. Glut1 was highly enriched in the sarcolemma, both in the basal state and after insulin treatment. Disruption of transverse tubule morphology by in vitro glycerol shock completely abolished insulin-stimulated glucose transport in isolated rat epitrochlearis muscles. These data indicate that: (a) Glut1 and Glut4 are targeted to distinct plasma membrane domains in skeletal muscle; (b) Glut1 contributes to basal transport at the sarcolemma and the bulk of insulin-stimulated transport is mediated by Glut4 localized in the transverse tubules; (c) insulin increases the apparent surface area of transverse tubules in skeletal muscle; and (d) insulin causes the unmasking of a COOH-terminal antigenic epitope in skeletal muscle in much the same fashion as it does in rat adipocytes.
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