ArticleThe Journal of cell biology1996
Plus-end motors override minus-end motors during transport of squid axon vesicles on microtubules.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
- Single-molecule fluorescence and in vivo optical traps: how multiple dyneins and kinesins interact.Chemical reviews · 2014Review
- Recycling of kinesin-1 motors by diffusion after transport.PloS one · 2013Article
- Two independent switches regulate cytoplasmic dynein's processivity and directionality.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Plus- and minus-end directed microtubule motors bind simultaneously to herpes simplex virus capsids using different inner tegument structures.PLoS pathogens · 2010Article
- No conventional function for the conventional kinesin?Traffic (Copenhagen, Denmark) · 2008Article
- Dynein and Star interact in EGFR signaling and ligand trafficking.Journal of cell science · 2008Article
- Cargo selection by specific kinesin light chain 1 isoforms.The EMBO journal · 2006Article
- Calsyntenin-1 docks vesicular cargo to kinesin-1.Molecular biology of the cell · 2006Article
- Coordinated transport of phosphorylated amyloid-beta precursor protein and c-Jun NH2-terminal kinase-interacting protein-1.The Journal of cell biology · 2005Article
- Local modulation of plus-end transport targets herpesvirus entry and egress in sensory axons.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Coordination of opposite-polarity microtubule motors.The Journal of cell biology · 2002Article
- Dynein, dynactin, and kinesin II's interaction with microtubules is regulated during bidirectional organelle transport.The Journal of cell biology · 2000Article
- Axonal membrane proteins are transported in distinct carriers: a two-color video microscopy study in cultured hippocampal neurons.Molecular biology of the cell · 2000Article
- Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport.Molecular biology of the cell · 1999Article
- Microtubule-based endoplasmic reticulum motility in Xenopus laevis: activation of membrane-associated kinesin during development.Molecular biology of the cell · 1999Article
- Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.Molecular biology of the cell · 1999Article
- The kinesin motor KIF3A is a component of the presynaptic ribbon in vertebrate photoreceptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Real-time imaging of the axonal transport of granules containing a tissue plasminogen activator/green fluorescent protein hybrid.Molecular biology of the cell · 1998Article
- KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles.Molecular biology of the cell · 1998Article
- Evidence for four cytoplasmic dynein heavy chain isoforms in rat testis.Molecular biology of the cell · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Plus- and minus-end vesicle populations from squid axoplasm were isolated from each other by selective extraction of the minus-end vesicle motor followed by 5'-adenylyl imidodiphosphate (AMP-PNP)-induced microtubule affinity purification of the plus-end vesicles. In the presence of cytosol containing both plus- and minus-end motors, the isolated populations moved strictly in opposite directions along microtubules in vitro. Remarkably, when treated with trypsin before incubation with cytosol, purified plus-end vesicles moved exclusively to microtubule minus ends instead of moving in the normal plus-end direction. This reversal in the direction of movement of trypsinized plus-end vesicles, in light of further observation that cytosol promotes primarily minus-end movement of liposomes, suggests that the machinery for cytoplasmic dynein-driven, minus-end vesicle movement can establish a functional interaction with the lipid bilayers of both vesicle populations. The additional finding that kinesin overrides cytoplasmic dynein when both are bound to bead surfaces indicates that the direction of vesicle movement could be regulated simply by the presence or absence of a tightly bound, plus-end kinesin motor; being processive and tightly bound, the kinesin motor would override the activity of cytoplasmic dynein because the latter is weakly bound to vesicles and less processive. In support of this model, it was found that (a) only plus-end vesicles copurified with tightly bound kinesin motors; and (b) both plus- and minus-end vesicles bound cytoplasmic dynein from cytosol.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.