ArticleThe EMBO journal1996
c-Jun stimulates origin-dependent DNA unwinding by polyomavirus large Tantigen.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- DNA-protein interaction dynamics at the Lamin B2 replication origin.Cell cycle (Georgetown, Tex.) · 2015Article
- Isolation and characterization of the ecdysone receptor and its heterodimeric partner ultraspiracle through development in Sciara coprophila.Chromosoma · 2013Article
- Back to the origin: reconsidering replication, transcription, epigenetics, and cell cycle control.Genes & cancer · 2012Article
- Characterization of sequence elements from Malvastrum yellow vein betasatellite regulating promoter activity and DNA replication.Virology journal · 2012Article
- A multiprotein complex necessary for both transcription and DNA replication at the β-globin locus.The EMBO journal · 2010Article
- Restriction of human polyomavirus BK virus DNA replication in murine cells and extracts.Journal of virology · 2009Article
- Genome-wide studies highlight indirect links between human replication origins and gene regulation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Activation of CREB/ATF sites by polyomavirus large T antigen.Journal of virology · 2005Article
- Replication of damaged DNA in vitro is blocked by p53.Nucleic acids research · 2003Article
- Inhibition of polyomavirus ori-dependent DNA replication by mSin3B.Journal of virology · 2002Article
- Stimulation of DNA replication from the polyomavirus origin by PCAF and GCN5 acetyltransferases: acetylation of large T antigen.Molecular and cellular biology · 2002Article
- Minute virus of mice initiator protein NS1 and a host KDWK family transcription factor must form a precise ternary complex with origin DNA for nicking to occur.Journal of virology · 2001Article
- DNA replication efficiency depends on transcription factor-binding sites.Journal of virology · 2001Article
- The dhfr oribeta-binding protein RIP60 contains 15 zinc fingers: DNA binding and looping by the central three fingers and an associated proline-rich region.Nucleic acids research · 2000Article
- The capacity of polyomavirus enhancer binding protein 2alphaB (AML1/Cbfa2) to stimulate polyomavirus DNA replication is related to its affinity for the nuclear matrix.Molecular and cellular biology · 1998Article
- Characterization of the DNA-binding domain of the bovine papillomavirus replication initiator E1.Journal of virology · 1998Article
- cis elements that contribute to geminivirus transcriptional regulation and the efficiency of DNA replication.Journal of virology · 1997Article
- The transcription activation domains of Fos and Jun induce DNA bending through electrostatic interactions.The EMBO journal · 1997Article
- Mechanisms of transcriptional regulation of cellular genes by SV40 large T- and small T-antigens.Virus genes · 1997Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The AP1 protein c-Jun has previously been shown to stimulate polyomavirus (Py) DNA replication in vivo. In order to define the mechanism, we added purified c-Jun protein to the origin-dependent and large T antigen (LT)-dependent in vitro DNA unwinding assay. c-Jun protein was found to stimulate by approximately 5-fold the unwinding of a 290 bp linear DNA fragment containing both the Py origin and the AP1 recognition sequence to which c-Jun binds. Efficient levels of stimulation were specifically observed at limiting concentrations of LT for unwinding. Under similar conditions, Py DNA replication was stimulated to a comparable extent by AP1 in a purified in vitro replication assay. Mobility shift and DNase I footprinting assays showed that c-Jun stimulates the ATP-dependent binding of LT to the origin core by approximately 7-fold. Furthermore, c-Jun was found to interact directly with LT, but not with replication protein A. The activities of c-Jun to stimulate unwinding and origin binding of LT were found to be harbored within the N-terminal region of c-Jun, which is distinct from the DNA binding domain. We speculate that certain transcription factors may possess specific DNA replication domains that function to stimulate the loading of replication factors at the origin during the initiation of DNA synthesis.
Indexed as
Identifiers
8896457PMC452308W2227799357What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.