ArticleThe EMBO journal1996
Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras Is Conserved Across Species.Genetics · 2016Article
- The 3' to 5' Exoribonuclease DIS3: From Structure and Mechanisms to Biological Functions and Role in Human Disease.Biomolecules · 2015Review
- Article
- The ribonuclease Dis3 is an essential regulator of the developmental transcriptome.BMC genomics · 2012Article
- Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility.Nature genetics · 2012Article
- Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.Yeast (Chichester, England) · 2011Article
- Drosophila melanogaster Dis3 N-terminal domains are required for ribonuclease activities, nuclear localization and exosome interactions.Nucleic acids research · 2010Article
- Dis3-like 1: a novel exoribonuclease associated with the human exosome.The EMBO journal · 2010Article
- Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast.Molecular and cellular biology · 2010Article
- Characterization of the Drosophila melanogaster Dis3 ribonuclease.Biochemical and biophysical research communications · 2009Article
- Interdependent nucleocytoplasmic trafficking and interactions of Dis3 with Rrp6, the core exosome and importin-alpha3.Traffic (Copenhagen, Denmark) · 2009Article
- Core exosome-independent roles for Rrp6 in cell cycle progression.Molecular biology of the cell · 2009Article
- The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome.Nucleic acids research · 2009Article
- Complex fate of paralogs.BMC evolutionary biology · 2008Article
- The exosome subunit Rrp44 plays a direct role in RNA substrate recognition.Molecular cell · 2007Article
- The lysogenic region of virus phiCh1: identification of a repressor-operator system and determination of its activity in halophilic Archaea.Extremophiles : life under extreme conditions · 2007Article
- Requirement of fission yeast Cid14 in polyadenylation of rRNAs.Molecular and cellular biology · 2006Article
- Differential distribution of exosome subunits at the nuclear lamina and in cytoplasmic foci.Molecular biology of the cell · 2006Article
- NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the two-hybrid method, we isolated a Saccharomyces cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis. Although Dis3sc has no homology to RanBP1, it bound directly to Ran and the S.cerevisiae Ran homologue Cnr1, but not to the S.cerevisiae RCC1 homologue Srm1. Upon binding to Ran with a 1:1 molar ratio, Dis3sc enhanced a nucleotide-releasing activity of RCC1 on Ran. In the presence of Dis3sc, the K(m) of RCC1 on Ran decreased by half, while the kcat was unchanged. In vivo, Dis3sp was present as oligomers of M(r) 670-200 kDa as previously reported, and the 200 kDa oligomer of Dis3sp was found to include Spi1 and Pim1, the S.pombe homologues of Ran and RCC1, respectively. Although the biological function of the heterotrimeric oligomer consisting of Dis3, Spi1 and Pim1 is unknown, our results indicate that Dis3 is a component of the RCC1-Ran pathway.
Indexed as
Identifiers
8896453PMC452304W1811811248What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.