ArticleThe EMBO journal1996
A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 70 citations in OpenAlex.
- The O-GlcNAcylation of β-actin Ser199 controls nuclear speckle localization and is dysregulated in diabetes.Histochemistry and cell biology · 2025Article
- Multi-locus genome-wide association study of fusarium head blight in relation to days to anthesis and plant height in a spring wheat association panel.Frontiers in plant science · 2023Article
- Hyperosmotic stress-induced microtubule disassembly in Chlamydomonas reinhardtii.BMC plant biology · 2022Article
- Article
- The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling.Genome biology and evolution · 2015Article
- A novel alpha kinase EhAK1 phosphorylates actin and regulates phagocytosis in Entamoeba histolytica.PLoS pathogens · 2014Article
- The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3.Cell research · 2012Article
- Dynamic microtubules are essential for efficient chromosome capture and biorientation in S. cerevisiae.The Journal of cell biology · 2006Article
- The dictyostelium kinome--analysis of the protein kinases from a simple model organism.PLoS genetics · 2006Article
- Article
- Molecular analysis of muskelin identifies a conserved discoidin-like domain that contributes to protein self-association.The Biochemical journal · 2004Article
- Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.Molecular biology of the cell · 2003Article
- Cloning and characterization of ectopically expressed transcripts for the actin-binding protein MIPP in mouse mammary carcinomas.Oncogene · 2001Article
- Analysis of HCF, the cellular cofactor of VP16, in herpes simplex virus-infected cells.Journal of virology · 2000Article
- Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain.Molecular biology of the cell · 1999Article
- The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domain.The EMBO journal · 1999Article
- HCF-dependent nuclear import of VP16.The EMBO journal · 1999Article
- Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiae.The Journal of cell biology · 1998Article
- Gelsolin and functionally similar actin-binding proteins are regulated by lysophosphatidic acid.The EMBO journal · 1998Article
- Muskelin, a novel intracellular mediator of cell adhesive and cytoskeletal responses to thrombospondin-1.The EMBO journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin-fragmin kinase (AFK) from Physarum polycephalum specifically phosphorylates actin in the EGTA-resistant 1:1 actin-fragmin complex. The cDNA deduced amino acid sequence reveals two major domains of approximately 35 kDa each that are separated by a hinge-like proline/serine-rich segment of 50 residues. Whereas the N-terminal domain does not show any significant similarity to protein sequences from databases, there are six complete kelch repeats in the protein that comprise almost the entire C-terminal half of the molecule. To prove the intrinsic phosphorylation activity of AFK, full-length or partial cDNA fragments were expressed both in a reticulocyte lysate and in Escherichia coli. In both expression systems, we obtained specific actin phosphorylation and located the catalytic domain in the N-terminal half. Interestingly, this region did not contain any of the known protein kinase consensus sequences. The only known sequence motif present that could have been involved in nucleotide binding was a nearly perfect phosphate binding loop (P-loop). However, introduction of two different point mutations into this putative P-loop sequence did not alter the catalytic activity of the kinase, which indicates an as yet unknown mechanism for phosphate transfer. Our data suggest that AFK belongs to a new class of protein kinases and that this actin phosphorylation might be the first example of a widely distributed novel type of regulation of the actin cytoskeleton in non-muscle cells.
Indexed as
Identifiers
8896448PMC452299W1899487985What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.