ArticleThe EMBO journal1996
Hoxb-2 transcriptional activation in rhombomeres 3 and 5 requires an evolutionarily conserved cis-acting element in addition to the Krox-20 binding site.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Transcriptional enhancers at 40: evolution of a viral DNA element to nuclear architectural structures.Trends in genetics : TIG · 2022Review
- Identification of a neural development gene expression signature in colon cancer stem cells reveals a role foriScience · 2022Article
- Neural stem cells deriving from chick embryonic hindbrain recapitulate hindbrain development in culture.Scientific reports · 2018Article
- Functional and Comparative Genomics of Hoxa2 Gene cis-Regulatory Elements: Evidence for Evolutionary Modification of Ancestral Core Element Activity.Journal of developmental biology · 2016Article
- The gene regulatory networks underlying formation of the auditory hindbrain.Cellular and molecular life sciences : CMLS · 2015Review
- Rostral and caudal pharyngeal arches share a common neural crest ground pattern.Development (Cambridge, England) · 2009Article
- Comparative analysis of Hox paralog group 2 gene expression during Nile tilapia (Oreochromis niloticus) embryonic development.Development genes and evolution · 2007Article
- Hoxb1 enhancer and control of rhombomere 4 expression: complex interplay between PREP1-PBX1-HOXB1 binding sites.Molecular and cellular biology · 2005Article
- Regulation of Hoxb2 by APL-associated PLZF protein.Oncogene · 2003Article
- Evolutionary conservation of regulatory elements in vertebrate Hox gene clusters.Genome research · 2003Article
- Regulatory gene networks and the properties of the developmental process.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Krox20 and kreisler co-operate in the transcriptional control of segmental expression of Hoxb3 in the developing hindbrain.The EMBO journal · 2002Article
- Analysis of a complete homeobox gene repertoire: implications for the evolution of diversity.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain.The EMBO journal · 1997Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Segmentation is a key feature of the development of the vertebrate hindbrain where it involves the generation of repetitive morphological units termed rhombomeres (r). Hox genes are likely to play an essential role in the specification of segmental identity and we have been investigating their regulation. We show here that the mouse and chicken Hoxb-2 genes are dependent for their expression in r3 and r5 on homologous enhancer elements and on binding to this enhancer of the r3/r5-specific transcriptional activator Krox-20. Among the three Krox-20 binding sites of the mouse Hoxb-2 enhancer, only the high-affinity site is absolutely necessary for activity. In contrast, we have identified an additional cis-acting element, Box1, essential for r3/r5 enhancer activity. It is conserved both in sequence and in position respective to the high-affinity Krox-20 binding site within the mouse and chicken enhancers. Furthermore, a short 44 bp sequence spanning the Box1 and Krox-20 sites can act as an r3/r5 enhancer when oligomerized. Box1 may therefore constitute a recognition sequence for another factor cooperating with Krox-20. Taken together, these data demonstrate the conservation of Hox gene regulation and of Krox-20 function during vertebrate evolution.
Indexed as
Identifiers
8895582PMC452281What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.