ArticleThe EMBO journal1996
The proteolysis of mitotic cyclins in mammalian cells persists from the end of mitosis until the onset of S phase.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
What it found
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Who cites it
116 citing papers in PubMed, 281 citations in OpenAlex.
- WEE1 opposes APC/CNature · 2026Article
- Temporal myc dynamics permit mitotic bypass, promoting polyploid phenotypes.Cancer letters · 2025Review
- Impact of Global Transcriptional Silencing on Cell Cycle Regulation and Chromosome Segregation in Early Mammalian Embryos.International journal of molecular sciences · 2021Review
- Bloom syndrome DNA helicase deficiency is associated with oxidative stress and mitochondrial network changes.Scientific reports · 2021Article
- Complex Cartography: Regulation of E2F Transcription Factors by Cyclin F and Ubiquitin.Trends in cell biology · 2020Review
- Co-regulation of the antagonistic RepoMan:Aurora-B pair in proliferating cells.Molecular biology of the cell · 2020Article
- Who guards the guardian? Mechanisms that restrain APC/C during the cell cycle.Biochimica et biophysica acta. Molecular cell research · 2018Review
- The E3 Ubiquitin Ligase SCF(Cyclin F) Transmits AKT Signaling to the Cell-Cycle Machinery.Cell reports · 2017Article
- Transcriptional landscape of the human cell cycle.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dual control by Cdk1 phosphorylation of the budding yeast APC/C ubiquitin ligase activator Cdh1.Molecular biology of the cell · 2016Article
- Human cytomegalovirus riding the cell cycle.Medical microbiology and immunology · 2015Review
- Degradation of Ndd1 by APC/C(Cdh1) generates a feed forward loop that times mitotic protein accumulation.Nature communications · 2015Article
- Co-activator independent differences in how the metaphase and anaphase APC/C recognise the same substrate.Biology open · 2014Article
- Strong expression of cyclin B2 mRNA correlates with a poor prognosis in patients with non-small cell lung cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Translation regulation and proteasome mediated degradation cooperate to keep stem-loop binding protein low in G1-phase.Journal of cellular biochemistry · 2014Article
- BRCA1 targets G2/M cell cycle proteins for ubiquitination and proteasomal degradation.Oncogene · 2013Article
- Role of cyclins in controlling progression of mammalian spermatogenesis.The International journal of developmental biology · 2013Review
- p53 can repress transcription of cell cycle genes through a p21(WAF1/CIP1)-dependent switch from MMB to DREAM protein complex binding at CHR promoter elements.Cell cycle (Georgetown, Tex.) · 2012Article
- Regulation of APC/C-Cdh1 and its function in neuronal survival.Molecular neurobiology · 2012Review
- Orphan kinases turn eccentric: a new class of cyclin Y-activated, membrane-targeted CDKs.Cell cycle (Georgetown, Tex.) · 2012Review
56 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied how the cell cycle-specific oscillations of mitotic B-type cyclins are generated in mouse fibroblasts. A reporter enzyme comprising the N-terminus of a B-type cyclin fused to bacterial chloramphenicol acetyl transferase (CAT) was degraded at the end of mitosis like endogenous cyclins. Point mutations in the destruction box of this construct completely abolished its mitotic instability. When the destructible reporter was driven by the cyclin B2 promoter, CAT activity mimicked the oscillations in the level of the endogenous cyclin B2. These oscillations were largely conserved when the reporter was transcribed constitutively from the SV40 promoter. Pulse-chase experiments or addition of the proteasome inhibitors lactacystin and ALLN showed that cyclin synthesis continued after the end of mitosis. The destruction box-specific degradation of cyclins normally ceases at the onset of S phase, and is active in fibroblasts arrested in G0 and in differentiated C2 myoblasts. We were able to reproduce this proteolysis in vitro in extracts of synchronized cells. Extracts of G1 cells degraded cyclin B1 whereas p27Kip1 was stable, in contrast, cyclin B1 remained stable and p27Kip1 was degraded in extracts of S phase cells.
Indexed as
Identifiers
8895573PMC452272W1457122365What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.