ArticleThe EMBO journal1996
The beta-amyloid domain is essential for axonal sorting of amyloid precursor protein.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 130 citations in OpenAlex.
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- The alteration and role of glycoconjugates in Alzheimer's disease.Frontiers in aging neuroscience · 2024Review
- Loss of Extrasynaptic Inhibitory Glycine Receptors in the Hippocampus of an AD Mouse Model Is Restored by Treatment with Artesunate.International journal of molecular sciences · 2023Article
- Lack of N-glycosylation increases amyloidogenic processing of the amyloid precursor protein.Glycobiology · 2022Article
- Implications of Glycosylation in Alzheimer's Disease.Frontiers in neuroscience · 2020Review
- Early Stage Glycosylation Biomarkers in Alzheimer's Disease.Medicines (Basel, Switzerland) · 2019Review
- Tau/MAPT disease-associated variant A152T alters tau function and toxicity via impaired retrograde axonal transport.Human molecular genetics · 2019Article
- Glycation vs. glycosylation: a tale of two different chemistries and biology in Alzheimer's disease.Glycoconjugate journal · 2016Review
- PAT1 inversely regulates the surface Amyloid Precursor Protein level in mouse primary neurons.BMC neuroscience · 2015Article
- UV irradiation accelerates amyloid precursor protein (APP) processing and disrupts APP axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Alzheimer's disease: pathophysiology and applications of magnetic nanoparticles as MRI theranostic agents.ACS chemical neuroscience · 2013Review
- Amyloid β precursor protein as a molecular target for amyloid β--induced neuronal degeneration in Alzheimer's disease.Neurobiology of aging · 2013Review
- Article
- Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.Biology open · 2012Article
- Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila.Molecular biology of the cell · 2012Article
- Trafficking and proteolytic processing of APP.Cold Spring Harbor perspectives in medicine · 2012Review
- Axonal transport of APP and the spatial regulation of APP cleavage and function in neuronal cells.Experimental brain research · 2012Review
- The amyloid precursor protein forms plasmalemmal clusters via its pathogenic amyloid-β domain.Biophysical journal · 2012Article
- Physiological functions of APP family proteins.Cold Spring Harbor perspectives in medicine · 2012Review
- Roles of Brain Angiotensin II in Cognitive Function and Dementia.International journal of hypertension · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analysed the axonal sorting signals of amyloid precursor protein (APP). Wild-type and mutant versions of human APP were expressed in hippocampal neurons using the Semliki forest virus system. We show that wild-type APP and mutations implicated in Alzheimer's disease and another brain beta-amyloidosis are sorted to the axon. By analysis of deletion mutants we found that the membrane-inserted APP ectodomain but not the cytoplasmic tail is required for axonal sorting. Systematic deletions of the APP ectodomain identified two regions required for axonal delivery: one encoded by exons 11-15 in the carbohydrate domain, the other encoded by exons 16-17 in the juxtamembraneous beta-amyloid domain. Treatment of the cells with the N-glycosylation inhibitor tunicamycin induced missorting of wild-type APP, supporting the importance of glycosylation in axonal sorting of APP. The data revealed a hierarchy of sorting signals on APP: the beta-amyloid-dependent membrane proximal signal was the major contributor to axonal sorting, while N-glycosylation had a weaker effect. Furthermore, recessive somatodendritic signals, most likely in the cytoplasmic tail, directed the protein to the dendrites when the ectodomain was deleted. Analysis of detergent solubility of APP and another axonally delivered protein, hemagglutinin, demonstrated that only hemagglutinin formed CHAPS-insoluble complexes, suggesting distinct mechanisms of axonal sorting for these two proteins. This study is the first delineation of sorting requirements of an axonally targeted protein in polarized neurons and indicates that the beta-amyloid domain plays a major role in axonal delivery of APP.
Indexed as
Identifiers
8895567PMC452266W1869777169What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.