Evidence map›Paper›PMID 8890247›Full record

ArticleInfection and immunity1996

Cloning, nucleotide sequence, and expression of the gene coding for a ribosome releasing factor-homologous protein of Brucella melitensis.

N Vizcaíno, A Cloeckaert, G Dubray, M S Zygmunt

Open access · bronzeAbstract read
In one paragraph

Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

N VizcaínoLaboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, Nouzilly, France. Nieves.Vizcaino@tours.inra.fr.
A Cloeckaert
G Dubray
M S Zygmunt
Infectiologie Animale et Santé Publique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The gene coding for a Brucella melitensis cytosoluble protein (CP24) that is immunogenic in infected sheep and a major component of brucellin INRA was cloned and sequenced. As in Brucella cells, CP24 was located in the cytoplasm of recombinant Escherichia coli. The amino acid sequence predicted from the cloned gene revealed 48 and 46% identity with the ribosome releasing factor, a protein factor required for release of the 70S ribosome from the mRNA, of E. coli and Haemophilus influenzae Rd, respectively. Sera from naturally infected sheep and sheep experimentally infected with B. melitensis H38 showed antibody reactivity against recombinant CP24.

Indexed as

ProteinsAmino Acid SequenceAnimalsAntibodies, BacterialBacterial ProteinsBase SequenceBrucella melitensisBrucellosisCloning, MolecularEscherichia coliGene ExpressionGenes, BacterialMolecular Sequence DataRecombinant ProteinsRibosomal ProteinsSequence AlignmentAntibodies, BacterialBacterial ProteinsCP24 protein, Brucella melitensisProteinsRecombinant ProteinsRibosomal Proteinsribosome releasing factor

Identifiers

PMID8890247
PMCPMC174453
OpenAlexW2100232902

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.