ArticleInfection and immunity1996
Attachment of a noninvasive enteric pathogen, enteropathogenic Escherichia coli, to cultured human intestinal epithelial monolayers induces transmigration of neutrophils.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Role of EspB in experimental human enteropathogenic Escherichia coli infection.Infection and immunity · 2000Trial
- Article
- Pathophysiology of enteropathogenicJournal of veterinary science · 2022Review
- EnteropathogenicInternational journal of molecular sciences · 2020Article
- EPEC NleH1 is significantly more effective in reversing colitis and reducing mortality than NleH2 via differential effects on host signaling pathways.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Goblet Cell Derived RELM-β Recruits CD4+ T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation.PLoS pathogens · 2015Article
- Pathogenic E. coli exploits SslE mucinase activity to translocate through the mucosal barrier and get access to host cells.PloS one · 2015Article
- Bacteroides fragilis subverts mucosal biology: from symbiont to colon carcinogenesis.The Journal of clinical investigation · 2014Review
- The structure and specificity of the type III secretion system effector NleC suggest a DNA mimicry mechanism of substrate recognition.Biochemistry · 2014Article
- In vitro coculture assay to assess pathogen induced neutrophil trans-epithelial migration.Journal of visualized experiments : JoVE · 2014Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Consuming transgenic goats' milk containing the antimicrobial protein lysozyme helps resolve diarrhea in young pigs.PloS one · 2013Article
- Enteroaggregative Escherichia coli promotes transepithelial migration of neutrophils through a conserved 12-lipoxygenase pathway.Cellular microbiology · 2012Article
- Polyethylene glycol diminishes pathological effects of Citrobacter rodentium infection by blocking bacterial attachment to the colonic epithelia.Gut microbes · 2011Article
- Article
- NleC, a type III secretion protease, compromises NF-κB activation by targeting p65/RelA.PLoS pathogens · 2010Article
- The clinical Pseudomonas fluorescens MFN1032 strain exerts a cytotoxic effect on epithelial intestinal cells and induces Interleukin-8 via the AP-1 signaling pathway.BMC microbiology · 2010Article
- The ex vivo response of human intestinal mucosa to enteropathogenic Escherichia coli infection.Cellular microbiology · 2009Article
- Vibrio cholerae flagellins induce Toll-like receptor 5-mediated interleukin-8 production through mitogen-activated protein kinase and NF-kappaB activation.Infection and immunity · 2008Article
- Flagellin-dependent and -independent inflammatory responses following infection by enteropathogenic Escherichia coli and Citrobacter rodentium.Infection and immunity · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
An intense inflammatory cell infiltrate, consisting primarily of polymorphonuclear leukocytes (PMN), accompanies enteric infection by enteropathogenic Escherichia coli (EPEC). The mechanism(s) by which this pathogen elicits PMN recruitment has not been studied. To determine whether EPEC infection of intestinal epithelial cells induces PMN to transmigrate, an in vitro model consisting of cultured human intestinal epithelial monolayers (T84), a human EPEC strain (E2348/69), and isolated human PMN was used. Results of these studies showed that EPEC attachment to T84 monolayers stimulated the transepithelial migration of PMN in a dose-dependent fashion. This event was not attributable to the classic bacterial chemoattractants, n-formylated peptides, or other soluble bacterial factors. A nonadherent EPEC strain, JPN15, was unable to cause PMN to cross the epithelial monolayer. Epithelial protein synthesis was required for maximum EPEC-induced PMN transmigration to occur. Transfer assays demonstrated the presence of a chemokine in sterilized medium from infected monolayers. Neutralizing antibodies to interleukin 8 ablated approximately 50% of the chemotactic activity. Studies with EPEC mutant strains revealed that the eaeB gene, required for the activation of signal transduction pathways in host cells, was crucial for eliciting PMN transmigration. These data show for the first time that attachment of a noninvasive enteric pathogen to intestinal epithelial cells induces PMN transmigration. These findings strongly suggest that EPEC attachment to target host cells activates a signal transduction cascade which ultimately leads to the expression and release of an epithelium-derived chemotactic factor(s) for PMN.
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