ArticleThe EMBO journal1996
The conserved mitotic kinase polo is regulated by phosphorylation and has preferred microtubule-associated substrates in Drosophila embryo extracts.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed.
- Testis- and ovary-expressed polo-like kinase transcripts and gene duplications affect male fertility when expressed in the Drosophila melanogaster germline.G3 (Bethesda, Md.) · 2025Article
- The Polo kinase Cdc5 is regulated at multiple levels in the adaptation response to telomere dysfunction.Genetics · 2023Article
- Cell Cycle Kinase Polo Is Controlled by a Widespread 3' Untranslated Region Regulatory Sequence in Drosophila melanogaster.Molecular and cellular biology · 2019Article
- The Centrioles, Centrosomes, Basal Bodies, and Cilia ofGenetics · 2017Review
- Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogaster.Open biology · 2014Article
- Drosophila SUUR protein associates with PCNA and binds chromatin in a cell cycle-dependent manner.Chromosoma · 2013Article
- Role of Survivin in cytokinesis revealed by a separation-of-function allele.Molecular biology of the cell · 2011Article
- Dissecting protein interactions during cytokinesis.Communicative & integrative biology · 2011Article
- The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts.The Journal of cell biology · 2010Article
- Regulation of centrosomes by the BRCA1-dependent ubiquitin ligase.Cancer biology & therapy · 2008Review
- NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus.The Journal of cell biology · 2007Article
- Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs.The EMBO journal · 2006Article
- Review
- Polo kinase and progression through M phase in Drosophila: a perspective from the spindle poles.Oncogene · 2005Review
- Structure and function of Polo-like kinases.Oncogene · 2005Review
- Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets.PLoS biology · 2004Article
- Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.The Journal of cell biology · 2003Article
- Plk phosphorylation regulates the microtubule-stabilizing protein TCTP.Molecular and cellular biology · 2002Article
- Cdc5 interacts with the Wee1 kinase in budding yeast.Molecular and cellular biology · 2001Article
- The polo-like kinase Plx1 is required for activation of the phosphatase Cdc25C and cyclin B-Cdc2 in Xenopus oocytes.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila gene polo encodes a protein kinase required for progression through mitosis. Wild-type polo protein migrates as a tight doublet of 67 kDa which is converted to a single band by phosphatase treatment, which also inactivates the kinase. We have determined putative polo substrates in a cell-free system derived from mutant embryos. Exogenous polo protein kinase phosphorylates proteins of sizes 220 kDa, 85 kDa and 54 kDa, to a greater extent when added to extracts of polo(1)-derived embryos compared with extracts of wild-type embryos, which in both cases have been subject to mild heat treatment to inactivate endogenous kinases. Proteins of the same size are predominantly phosphorylated by the endogenous kinases present in wild-type extracts, and are either not phosphorylated or are poorly phosphorylated in extracts of polo(1)-derived embryos. We show that a specific monoclonal antibody to beta-tubulin precipitates the phosphorylated 54 kDa protein together with an associated 85 kDa protein also phosphorylated by polo protein kinase. Moreover polo binds to an 85 kDa protein which is enriched in microtubule preparations. We discuss the extent to which these in vitro phosphorylation results reflect the effects of mutations in polo on microtubule behaviour during the mitotic cycle.
Indexed as
Identifiers
8890161PMC452225What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.