ArticleBiophysical journal1996
Calcium alone does not fully activate the thin filament for S1 binding to rigor myofibrils.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 81 citations in OpenAlex.
- Troponin Structural Dynamics in the Native Cardiac Thin Filament Revealed by Cryo Electron Microscopy.Journal of molecular biology · 2024Article
- Mouse Models of Cardiomyopathies Caused by Mutations in Troponin C.International journal of molecular sciences · 2023Review
- Cryo-Electron Microscopy Reveals Cardiac Myosin Binding Protein-C M-Domain Interactions with the Thin Filament.Journal of molecular biology · 2022Article
- cMyBP-C phosphorylation modulates the time-dependent slowing of unloaded shortening in murine skinned myocardium.The Journal of general physiology · 2021Article
- Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables.Molecular biology of the cell · 2014Article
- The myosin duty ratio tunes the calcium sensitivity and cooperative activation of the thin filament.Biochemistry · 2013Article
- Tropomyosin and myosin-II cellular levels promote actomyosin ring assembly in fission yeast.Molecular biology of the cell · 2010Article
- Article
- Differences between cardiac and skeletal troponin interaction with the thin filament probed by troponin exchange in skeletal myofibrils.Biophysical journal · 2009Article
- Phosphorylation of tropomyosin extends cooperative binding of myosin beyond a single regulatory unit.Cell motility and the cytoskeleton · 2009Article
- Tropomyosin dynamics in cardiac thin filaments: a multisite forster resonance energy transfer and anisotropy study.Biophysical journal · 2008Article
- Myofibrillar troponin exists in three states and there is signal transduction along skeletal myofibrillar thin filaments.Journal of molecular biology · 2006Article
- Ablation of myosin-binding protein-C accelerates force development in mouse myocardium.Biophysical journal · 2006Article
- Physiological consequences of thin filament cooperativity for vertebrate striated muscle contraction: a theoretical study.Journal of muscle research and cell motility · 2006Article
- Thin filament remodeling in failing myocardium.Heart failure reviews · 2005Review
- Arginine-glycine-aspartic acid (RGD)-containing peptides inhibit the force production of mouse papillary muscle bundles via alpha 5 beta 1 integrin.The Journal of physiology · 2005Article
- Roles of phosphorylation of myosin binding protein-C and troponin I in mouse cardiac muscle twitch dynamics.The Journal of physiology · 2004Article
- Unloaded shortening velocity in single permeabilized vascular smooth muscle cells is independent of microtubule status.Journal of muscle research and cell motility · 2004Article
- Contractile effects of the exchange of cardiac troponin for fast skeletal troponin in rabbit psoas single myofibrils.The Journal of physiology · 2003Article
- Activation of the calcium-regulated thin filament by myosin strong binding.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Skeletal muscle contraction is regulated by calcium via troponin and tropomyosin and appears to involve cooperative activation of cross-bridge binding to actin. We studied the regulation of fluorescent myosin subfragment 1 (fS1) binding to rigor myofibrils over a wide range of fS1 and calcium levels using highly sensitive imaging techniques. At low calcium and low fS1, the fluorescence was restricted to the actin-myosin overlap region. At high calcium and very low fS1, the fluorescence was still predominantly in the overlap region. The ratio of nonoverlap to overlap fluorescence intensity showed that increases in the fS1 level resulted in a shift in maximum fluorescence from the overlap to the nonoverlap region at both low and high calcium; this transition occurred at lower fS1 levels in myofibrils with high calcium. At a fixed fS1 level, increases in calcium also resulted in a shift in maximum fluorescence from the overlap region to the nonoverlap region. These results suggest that calcium alone does not fully activate the thin filament for rigor S1 binding and that, even at high calcium, the thin filament is not activated along its entire length.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.