ArticleBiophysical journal1996
Kinematics of red cell aspiration by fluorescence-imaged microdeformation.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Energy Dissipation in the Human Red Cell Membrane.Biomolecules · 2023Article
- αI-spectrin represents evolutionary optimization of spectrin for red blood cell deformability.Biophysical journal · 2021Article
- On the Role of Curved Membrane Nanodomains, and Passive and Active Skeleton Forces in the Determination of Cell Shape and Membrane Budding.International journal of molecular sciences · 2021Article
- Theoretical Bases for the Role of Red Blood Cell Shape in the Regulation of Its Volume.Frontiers in physiology · 2020Review
- Liquid Metal-Based Multifunctional Micropipette for 4D Single Cell Manipulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling.Scientific reports · 2017Article
- A novel strain energy relationship for red blood cell membrane skeleton based on spectrin stiffness and its application to micropipette deformation.Biomechanics and modeling in mechanobiology · 2016Article
- Molecular mechanisms of cellular mechanosensing.Nature materials · 2013Article
- Stress-free state of the red blood cell membrane and the deformation of its skeleton.Cellular & molecular biology letters · 2012Article
- Native ultrastructure of the red cell cytoskeleton by cryo-electron tomography.Biophysical journal · 2011Article
- Multiscale simulation of erythrocyte membranes.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Diffusion in a fluid membrane with a flexible cortical cytoskeleton.Biophysical journal · 2009Article
- Integral protein linkage and the bilayer-skeletal separation energy in red blood cells.Biophysical journal · 2008Article
- Mechanical properties of the cell nucleus and the effect of emerin deficiency.Biophysical journal · 2006Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Power-law rheology of isolated nuclei with deformation mapping of nuclear substructures.Biophysical journal · 2005Article
- Topographical pattern dynamics in passive adhesion of cell membranes.Biophysical journal · 2004Article
- Deformation-enhanced fluctuations in the red cell skeleton with theoretical relations to elasticity, connectivity, and spectrin unfolding.Biophysical journal · 2001Article
- Statistical thermodynamics of membrane bending-mediated protein-protein attractions.Biophysical journal · 2001Article
- Actin protofilament orientation in deformation of the erythrocyte membrane skeleton.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Maps of fluorescing red cell membrane components on a pipette-aspirated projection are quantitated in an effort to elucidate and unify the heterogeneous kinematics of deformation. Transient gradients of diffusing fluorescent lipid first demonstrate the fluidity of an otherwise uniform-density bilayer and corroborate a "universal" calibration scale for relative surface density. A steep but smooth and stable gradient in the densities of the skeleton components spectrin, actin, and protein 4.1 is used to estimate large elastic strains along the aspirated skeleton. The deformation fields are argued to be an unhindered response to loading in the surface normal direction. Density maps intermediate to those of the compressible skeleton and fluid bilayer are exhibited by particular transmembrane proteins (e.g., Band 3) and yield estimates for the skeleton-connected fractions. Such connected proteins appear to occupy a significant proportion of the undeformed membrane surface and can lead to steric exclusion of unconnected integral membrane proteins from regions of network condensation. Consistent with membrane repatterning kinematics in reversible deformation, final vesiculation of the projection tip produces a cell fragment concentrated in freely diffusing proteins but depleted of skeleton.
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Registered trials
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