ArticleMolecular and cellular biology1996
A potential role for Elf-1 in terminal transferase gene regulation.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- IKAROS Family Transcription Factors in Lymphocyte Differentiation and Function.Advances in experimental medicine and biology · 2024Review
- Cellular signaling and epigenetic regulation of gene expression in leukemia.Advances in biological regulation · 2020Review
- Gene expression is implicated in the ability of pikas to occupy Himalayan elevational gradient.PloS one · 2018Article
- Article
- Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription.Biochimica et biophysica acta · 2016Article
- Protein phosphatase 1 (PP1) and Casein Kinase II (CK2) regulate Ikaros-mediated repression of TdT in thymocytes and T-cell leukemia.Pediatric blood & cancer · 2014Article
- Ikaros and tumor suppression in acute lymphoblastic leukemia.Critical reviews in oncogenesis · 2011Review
- Recruitment of ikaros to pericentromeric heterochromatin is regulated by phosphorylation.The Journal of biological chemistry · 2008Article
- Down-regulation of TDT transcription in CD4(+)CD8(+) thymocytes by Ikaros proteins in direct competition with an Ets activator.Genes & development · 2001Article
- Binding of Ikaros to the lambda5 promoter silences transcription through a mechanism that does not require heterochromatin formation.The EMBO journal · 2001Article
- MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein.Molecular and cellular biology · 2001Article
- Ets target genes: past, present and future.Oncogene · 2000Review
- An ikaros-containing chromatin-remodeling complex in adult-type erythroid cells.Molecular and cellular biology · 2000Article
- Interleukin-2 (IL-2) regulates the accessibility of the IL-2-responsive enhancer in the IL-2 receptor alpha gene to transcription factors.Molecular and cellular biology · 1999Article
- Helios, a T cell-restricted Ikaros family member that quantitatively associates with Ikaros at centromeric heterochromatin.Genes & development · 1998Article
- Sp1 activation of a TATA-less promoter requires a species-specific interaction involving transcription factor IID.Nucleic acids research · 1998Article
- Hematopoietic stem cells and lymphoid progenitors express different Ikaros isoforms, and Ikaros is localized to heterochromatin in immature lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Elf-1 contributes to the function of the complex interleukin (IL)-2-responsive enhancer in the mouse IL-2 receptor alpha gene.The Journal of experimental medicine · 1997Article
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7 authors at 2 institutions in 1 country.
Funding
Abstract
The terminal deoxynucleotidyltransferase (TdT) gene represents an attractive model for the analysis of gene regulation during an early phase of lymphocyte development. In previous studies, we identified a DNA element, termed D', which is essential for TdT promoter activity in immature lymphocytes, and two classes of D'-binding factors, Ikaros proteins and Ets proteins. Here, we report a detailed mutant analysis of the D' element which suggests that an Ets protein, rather than an Ikaros protein, activates TdT transcription. Since multiple Ets proteins are expressed in developing lymphocytes and are capable of binding to the D' element, DNA affinity chromatography was used to determine if one of the Ets proteins might bind to the D' element with a uniquely high affinity, thereby implicating that protein as a potential TdT activator. Indeed, one binding activity was greatly enriched in the high-salt eluates from a D' affinity column. Peptide microsequencing revealed that the enriched protein was Elf-1. Immunoblot analyses confirmed that in nuclear extracts, Elf-1 has a significantly higher affinity for the D' sequence than does another Ets protein, Ets-1. Transactivation and expression studies support the hypothesis that Elf-1 activates TdT transcription in immature T and B cells. Finally, a D' mutation which selectively reduces Elf-1 binding, but not the binding of other Ets proteins, was found to greatly reduce TdT promoter activity. Although Elf-1 previously had been implicated in the inducible activation of genes in mature T and B cells, our results suggest that it also plays an important role in regulating genes during an early phase of lymphocyte development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.