ArticleMolecular and cellular biology1996
Growth and differentiation of C2 myogenic cells are dependent on serum response factor.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- PAX7, a Key for Myogenesis Modulation in Muscular Dystrophies through Multiple Signaling Pathways: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- A Multiomics Approach to Identify Genes Associated with Childhood Asthma Risk and Morbidity.American journal of respiratory cell and molecular biology · 2017Trial
- Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles.Frontiers in physiology · 2021Article
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- Srf controls satellite cell fusion through the maintenance of actin architecture.The Journal of cell biology · 2018Article
- Botulinum toxin A-induced muscle paralysis stimulates Hdac4 and differential miRNA expression.PloS one · 2018Article
- P311, Friend, or Foe of Tissue Fibrosis?Frontiers in pharmacology · 2018Review
- miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.The Journal of experimental biology · 2017Article
- Histone methyltransferase Setd2 is critical for the proliferation and differentiation of myoblasts.Biochimica et biophysica acta. Molecular cell research · 2017Article
- Reduced nuclear translocation of serum response factor is associated with skeletal muscle atrophy in a cigarette smoke-induced mouse model of COPD.International journal of chronic obstructive pulmonary disease · 2017Article
- Noncoding RNAs in the regulation of skeletal muscle biology in health and disease.Journal of molecular medicine (Berlin, Germany) · 2016Review
- Overexpression of Striated Muscle Activator of Rho Signaling (STARS) Increases C2C12 Skeletal Muscle Cell Differentiation.Frontiers in physiology · 2016Article
- Differential microRNA Expression in Fast- and Slow-Twitch Skeletal Muscle of Piaractus mesopotamicus during Growth.PloS one · 2015Article
- Article
- MicroRNA-199a is induced in dystrophic muscle and affects WNT signaling, cell proliferation, and myogenic differentiation.Cell death and differentiation · 2013Article
- miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors.Orphanet journal of rare diseases · 2013Article
- Locally expressed IGF1 propeptide improves mouse heart function in induced dilated cardiomyopathy by blocking myocardial fibrosis and SRF-dependent CTGF induction.Disease models & mechanisms · 2012Article
- MASTR directs MyoD-dependent satellite cell differentiation during skeletal muscle regeneration.Genes & development · 2012Article
- Protein tyrosine phosphatase-like A regulates myoblast proliferation and differentiation through MyoG and the cell cycling signaling pathway.Molecular and cellular biology · 2012Article
- Serum response factor is required for cortical axon growth but is dispensable for neurogenesis and neocortical lamination.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to study to what extent and at which stage serum response factor (SRF) is indispensable for myogenesis, we stably transfected C2 myogenic cells with, successively, a glucocorticoid receptor expression vector and a construct allowing for the expression of an SRF antisense RNA under the direction of the mouse mammary tumor virus long terminal repeat. In the clones obtained, SRF synthesis is reversibly down-regulated by induction of SRF antisense RNA expression by dexamethasone, whose effect is antagonized by the anti-hormone RU486. Two kinds of proliferation and differentiation patterns have been obtained in the resulting clones. Some clones with a high level of constitutive SRF antisense RNA expression are unable to differentiate into myotubes; their growth can be blocked by further induction of SRF antisense RNA expression by dexamethasone. Other clones are able to differentiate and are able to synthesize SRF, MyoD, myogenin, and myosin heavy chain at confluency. When SRF antisense RNA expression is induced in proliferating myoblasts by dexamethasone treatment, cell growth is blocked and cyclin A concentration drops. When SRF antisense RNA synthesis is induced in arrested confluent myoblasts cultured in a differentiation medium, cell fusion is blocked and synthesis of not only SRF but also MyoD, myogenin, and myosin heavy chain is inhibited. Our results show, therefore, that SRF synthesis is indispensable for both myoblast proliferation and myogenic differentiation.
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