ArticleMolecular and cellular biology1996
A suboptimal 5' splice site is a cis-acting determinant of nuclear export of polyomavirus late mRNAs.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- LENG8 mediates RNA nuclear retention and degradation in eukaryotes.Molecular cell · 2026Article
- Coordinating mRNA maturation: The U1 relay model.Molecular cell · 2026Review
- Localization of RNAs in the nucleus:RNA biology · 2021Review
- Let It Go: HIV-1Journal of virology · 2021Review
- Strength in Diversity: Nuclear Export of Viral RNAs.Viruses · 2020Review
- Article
- Hidden regulation of herpes simplex virus 1 pre-mRNA splicing and polyadenylation by virally encoded immediate early gene ICP27.PLoS pathogens · 2019Article
- Review
- Identification of cis-Regulatory Elements in the dmyc Gene of Drosophila Melanogaster.Gene regulation and systems biology · 2012Article
- Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF.Molecular and cellular biology · 2010Article
- RNA processing in the polyoma virus life cycle.Frontiers in bioscience (Landmark edition) · 2009Review
- UAP56 levels affect viability and mRNA export in Caenorhabditis elegans.RNA (New York, N.Y.) · 2003Article
- Reduction of target gene expression by a modified U1 snRNA.Molecular and cellular biology · 2001Article
- The sequence complementarity between HIV-1 5' splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA.RNA (New York, N.Y.) · 2001Article
- Posttranslational phosphorylation and ubiquitination of the Saccharomyces cerevisiae Poly(A) polymerase at the S/G(2) stage of the cell cycle.Molecular and cellular biology · 2000Article
- An exon that prevents transport of a mature mRNA.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.The EMBO journal · 1999Article
- A premature termination codon interferes with the nuclear function of an exon splicing enhancer in an open reading frame-dependent manner.Molecular and cellular biology · 1999Article
- Intron retention may regulate expression of Epstein-Barr virus nuclear antigen 3 family genes.Journal of virology · 1999Article
- Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.The EMBO journal · 1998Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mouse polyomavirus has been used as a model system to study nucleocytoplasmic transport of mRNA. Three late mRNAs encoding the viral capsid proteins are generated by alternative splicing from common pre-mRNA molecules. mRNAs encoding the virion protein VP2 (mVP2) harbor an unused 5' splice site, and more than half of them remain fully unspliced yet are able to enter the cytoplasm for translation. Examination of the intracellular distribution of late viral mRNAs revealed, however, that mVP2 molecules are exported less efficiently than are mVP1 and mVP3, in which the 5' splice site has been removed by splicing. Point mutations and deletion analyses demonstrated that the efficiency of mVP2 export is inversely correlated with the strength of the 5' splice site and that unused 3' splice sites present in the mRNA have little or no effect on export. These results suggest that the unused 5' splice site is a key player in mVP2 export. Interestingly, mRNAs carrying large deletions but retaining the 5' splice site exhibited a wild-type mVP2 export phenotype, suggesting that there are no other constitutive cis-acting sequences involved in mVP2 export. RNA stability measurements confirmed that the subcellular distribution differences between these mRNAs were not due to differential half-lives between the two cellular compartments. We therefore conclude that the nuclear export of mVP2 is strongly influenced by a suboptimal 5' splice site. Furthermore, results comparing spliced and unspliced forms of mVP2 molecules indicated that the process of splicing does not enhance nuclear export. Since mVP2 and some of its mutant forms can accumulate in the cytoplasm in the absence of splicing, we propose that splicing is not a prerequisite for mRNA export in the polyomavirus system; rather, removal of splicing machinery from mRNAs may be required. The possibility that export of other viral mRNAs can be influenced by suboptimal splicing signals is also discussed.
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